PROTEOMICS AND BIOINFORMATICS CORE

华盛顿大学糖尿病研究中心的蛋白质组学和生物信息学核心提供了 加入调查员现代质谱（MS）和复杂的数据集分析的强大工具。的 核心的目标是：（1）执行MS，以无偏地发现和半定量分析复杂的 使用最先进的非靶向和靶向LC-MS/MS技术对蛋白质样品进行定性和 定量分析，包括开发用于结构鉴定的新MS方法;（2）提供 经验证的与糖尿病相关的特定蛋白质分析物的靶向定量测定， 翻译研究;（3）协助DRC分支机构进行研究的实验设计，以确保成功 蛋白质组学分析，并提供MS原理和使用方面的培训;（4）提供生物信息学分析 (e.g.,差异表达分析、蛋白质-蛋白质相互作用网络分析和途径富集 蛋白质组学和转录组学数据集，包括RNA-seq和单细胞RNA-seq，目的是 提供一个综合的，系统生物学概述糖尿病和糖尿病相关的疾病过程。通过 提供进行最先进的蛋白质组学分析所需的集中设施和专业知识， 定量质谱分析和生物信息学分析，核心符合这些目标， 效率和成本效益，以支持基础和临床研究。此外，通过集中和 标准化程序，蛋白质组学和生物信息学核心为其附属研究人员提供了一个共同的 一套分析工具，用于获得对参与的分子机制的统一理解 糖尿病及其相关并发症的病理生理过程。

The Proteomics and Bioinformatics Core of the University of Washington Diabetes Research Center provides affiliate investigators the powerful tools of modern mass spectrometry (MS) and complex data set analysis. The goals of the Core are to: (1) Perform MS for unbiased discovery and semi-quantitative analyses of complex protein samples using state-of-the-art untargeted and targeted LC-MS/MS techniques for both qualitative and quantitative analysis, including development of new MS methods for structural identification; (2) Provide validated targeted quantitative assays of specific protein analytes relevant to diabetes in support of clinical and translational studies; (3) Assist DRC affiliates with experimental design of their studies to ensure successful proteomics analysis and provide training in principles and use of MS; and (4) Provide bioinformatics analysis (e.g., differential expression analysis, protein-protein interaction network analysis, and pathway enrichment analysis) for proteomic and transcriptomic data sets, including RNA-seq and single cell RNA-seq, with the aim of providing an integrated, systems biology overview of diabetes and diabetes-related disease processes. By providing a centralized facility and expertise necessary to perform state-of-the-art proteomics analysis, quantitative mass spectrometric assays, and bioinformatics analysis, the Core meets these goals with optimal efficiency and cost-effectiveness in support of basic and clinical research. Further, by centralizing and standardizing procedures, the Proteomics and Bioinformatics Core provides its affiliate investigators a common set of analytical tools for obtaining a unified understanding of molecular mechanisms involved in pathophysiologic processes of diabetes and its associated complications.