Subcellular localization of nanoparticles

描述（由申请人提供）：该计划的总体目标是研究具有明确物理化学性质的不同类别和亚型纳米颗粒（NPs）的细胞摄取、运输和亚细胞定位，以创建一个参考表，该参考表将NPs的亚细胞分布与其内在物理化学性质联系起来，跨越一系列细胞系。NPs的亚细胞命运与NPs的治疗效果和生物安全性有关。在血清调理素存在的情况下，纳米材料的大小、形状、电荷和化学成分对细胞进入和随后的亚细胞定位的有效影响将被研究。该项目的预期结果是创建一个参考表，通过快速扩展我们对材料内在特性对其细胞内目的地的影响的认识，加速纳米材料从实验室到临床的过渡。最终成果是NP及其亚细胞位置的综合表，将指导NP药物传递系统的未来发展，以快速扩展生物医学应用，包括癌症治疗、心血管成像和基因治疗。

DESCRIPTION (provided by applicant): The overall goal of this initiative is to investigate the cellular uptake, trafficking, and sub-cellular localization of different classes and subtypes of nanoparticles (NPs) with well-defined physiochemical properties for the creation of a reference table that relates the sub-cellular distribution of NPs to their intrinsic physiochemical properties across a range of cell lines. The subcellular fate of NPs is relevant both in terms of the therapeutic efficacy and biosafety of the NPs. The effective impact of size, shape, charge, and chemical composition of nanomaterials, in the presence of serum opsonins, on both cellular entry and subsequent subcellular localization will be investigated. The expected outcome of this project is to create a reference table that accelerates the transition of nanomaterials from the bench to the clinic by rapidly expanding our knowledge of the effect of a material's intrinsic characteristics on its intracellular destination. The final product, a comprehensive table of NPs and their subcellular locations, will guide the future development of NP drug delivery systems for rapid expansion of biomedical applications, including cancer therapy, cardiovascular imaging, and gene therapy. PUBLIC HEALTH RELEVANCE: What this project seeks to deliver is a multi-dimensional reference table that relates the subcellular distribution and toxicity of NPs to their intrinsic physiochemical properties across a range of diverse cells and cell lines. It is our hope that the data generated from this project will serve as a resource for future research and encourage model development and new insights into nanotechnologies for imaging and drug delivery.