Ultraspec III TOF/TOF 200 MALDI-TOF Mass Spectrometry Imaging System

说明(申请人提供)：申请资金用于购买Ultraflex III TOF/TOF 200 MALDI-TOF质谱仪成像系统、最新的Ultraflex III TOF/TOF200模块化飞行时间质谱仪以及相应的软件和附件，以便为组织分子成像提供样品准备。质谱学成像系统也将用于蛋白质鉴定，但主要是对参照组织(对照组织)和被调查组织(主要来自主要用户组)之间不同蛋白质表达的分子成像和检测。分子成像和蛋白质识别系统将极大地有利于七名由NIH资助的研究人员的研究和教学努力，他们的项目在本意见书中有详细介绍。布朗大学校园调查人员和附属于布朗大学沃伦·阿尔伯特医学院的五家生命医院也将获得这种仪器。该仪器将安装在蛋白质组学核心中，该核心是NIH/NCRR资助的生物医学卓越研究中心(Cobre)癌症研究开发中心(CCRD)的一部分，也将被Cobre CCRD研究人员使用。两名马克案调查人员(希克森博士和莫斯博士)也是科布雷CCRD项目的成员。蛋白质组学核心实验室位于核心研究实验室(CRL)附近。计划在蛋白质组学核心和CRL之间进行密切合作，以促进MALDI TOF/TOF系统在分子成像方面的最佳使用。CRL实验室及其主任Paul McMillan博士在组织化学和分子成像样品制备方面的丰富经验也将为这一最先进仪器的用户提供非凡的资源。目前蛋白质组学核心中有两个质谱仪在使用。首先，QSTAR XL(应用生物系统/SCHEX)在电泳法分离和凝胶内消化后，或在更复杂的样品，如细胞和细胞器，特别是质膜提取物的凝胶内消化后，正被用于蛋白质鉴定。第二个是Ciphergen/Bio-Rad SELDI-TOF系统，用于生物标记物发现中的蛋白质图谱。这些仪器成功地为80多名客户提供了服务，这些客户大多来自LIFESPAN和布朗大学科学界，但也有来自全美其他学术界的其他客户。Ultraflex III TOF/TOF 200 MALDI-TOF质谱学成像系统将补充和增强蛋白质组学核心质谱学服务，从而为科学界提供以多种方式分析蛋白质的灵活性和广度，最终目标是发现疾病生物标记物和细胞分化的生物标记物。我们的意图是，核心用户的这项技术的研究成果最终将加快与蛋白质组相关的公共卫生研究：所请求的仪器不仅将支持本申请中描述的七个主要用户组项目的翻译和基础科学研究，还将支持五个生化研究卓越中心(COBRE)的研究人员，包括我们的中心(Cobre CCRD)、罗德岛医院的Cobre骨骼健康中心和布朗大学、妇女和婴儿医院的Cobre中心和罗杰·威廉姆斯医学中心，并可能使来自布朗大学和6家附属教学医院的其他研究人员受益。最后，Ultraflex III TOF/TOF 200 MALDI-TOF质谱学成像系统将是罗德岛州这种配置和这种应用的唯一仪器，可以向整个科学界开放。

DESCRIPTION (provided by applicant): Funds are requested for the acquisition of Ultraflex III TOF/TOF 200 MALDI- TOF Mass Spectrometry Imaging System, an up-to-date Ultraflex III TOF/TOF200 modular time-of-flight mass spectrometer together with corresponding software and accessories, to provide sample preparation for tissue molecular imaging. The mass spectrometry imaging system will also be used for protein identification, but the main propose will be the molecular imaging and detection in different protein expression between the reference (control tissue) and investigated tissue, mainly from the Major User Group (MUG). The system for molecular imaging and protein identification will significantly benefit the research and teaching endeavors of the seven NIH-funded investigators whose projects are detailed in this submission. Brown University campus- based investigators and of the five Lifespan Hospitals that are affiliated with the Brown University's Warren Alpert Medical School will also have access to this instrument. This instrument will be located in the Proteomics Core, which is part of the NIH/NCRR-funded Centers of Biomedical Research Excellence (COBRE) Center for Cancer Research Development (CCRD), and will be also used by the COBRE CCRD investigators. Two of the MUG investigators (Drs. Hixson and Moss) are also members of the COBRE CCRD program. Proteomics Core laboratory is located adjacent to Core Research Laboratories (CRL). A tight collaboration between the Proteomics Core and CRL is planned to foster optimal use of the MALDI TOF/TOF system for molecular imaging. The vast experience of the CRL laboratory and its Director, Dr. Paul McMillan, both in histochemistry and sample preparation for molecular imaging will also be an extraordinary resource to the users of this state-of-the-art instrument. Currently there are two mass spectrometers in use in the Proteomics Core. First, the QSTAR XL (Applied Biosystems/Sciex) is being used for protein identification after electrophoretic separation and in-gel digestion, or after in-gel digestion of more complex samples such as cell and organelles, especially plasma membrane extracts. The second one is the Ciphergen/Bio-Rad SELDI-TOF system, which is used for protein profiling in biomarker discovery. These instruments successfully serve a group of more than 80 costumers mostly from the Lifespan and Brown University scientific community, but also other customers from other academic communities throughout the United States. The Ultraflex III TOF/TOF 200 MALDI-TOF Mass Spectrometry Imaging System will complement and enhance the Proteomics Core mass spectrometry services, thus providing the scientific community with the flexibility and breadth to analyze proteins in numerous ways with the final goal to discover disease biomarkers and biomarkers for cellular differentiation. It is our intent that Core users' research findings from this technology will ultimately expedite proteomics-related translational research PUBLIC HEALTH RELEVANCE: The requested instrumentation will not only support the translational and basic science research of the seven Major User Group projects described in this application, but will also support investigators of five Center of Biochemical Research Excellence (COBRE), including our Center (COBRE CCRD), COBRE Center for Skeletal Health at the Rhode Island Hospital and COBRE Centers at the Brown University, Women and Infants Hospital and Roger Williams Medical Center, and will potentially benefit other investigators from Brown University and 6 affiliated teaching hospitals. Finally, the Ultraflex III TOF/TOF 200 MALDI-TOF Mass Spectrometry Imaging System will be the only instrument in this configuration and for this kind of application in the State of Rhode Island, with open access to the whole scientific community.