Subcellular localization of nanoparticles

描述（由申请方提供）：该计划的总体目标是研究具有明确理化性质的不同类别和亚型纳米颗粒（NP）的细胞摄取、运输和亚细胞定位，以创建一个参考表，将NP的亚细胞分布与其在一系列细胞系中的内在理化性质联系起来。NP的亚细胞命运在NP的治疗功效和生物安全性方面都是相关的。将研究纳米材料的大小、形状、电荷和化学成分在血清调理素存在下对细胞进入和随后的亚细胞定位的有效影响。该项目的预期成果是创建一个参考表，通过快速扩展我们对材料内在特性对其细胞内目的地的影响的了解，加速纳米材料从实验室到临床的过渡。最终产品，一个全面的表纳米粒子及其亚细胞位置，将指导未来的发展纳米药物输送系统的快速扩展的生物医学应用，包括癌症治疗，心血管成像和基因治疗。 公共卫生相关性：该项目旨在提供一个多维参考表，将纳米颗粒的亚细胞分布和毒性与其在一系列不同细胞和细胞系中的内在理化性质联系起来。我们希望该项目产生的数据将作为未来研究的资源，并鼓励模型开发和对成像和药物输送纳米技术的新见解。

DESCRIPTION (provided by applicant): The overall goal of this initiative is to investigate the cellular uptake, trafficking, and sub-cellular localization of different classes and subtypes of nanoparticles (NPs) with well-defined physiochemical properties for the creation of a reference table that relates the sub-cellular distribution of NPs to their intrinsic physiochemical properties across a range of cell lines. The subcellular fate of NPs is relevant both in terms of the therapeutic efficacy and biosafety of the NPs. The effective impact of size, shape, charge, and chemical composition of nanomaterials, in the presence of serum opsonins, on both cellular entry and subsequent subcellular localization will be investigated. The expected outcome of this project is to create a reference table that accelerates the transition of nanomaterials from the bench to the clinic by rapidly expanding our knowledge of the effect of a material's intrinsic characteristics on its intracellular destination. The final product, a comprehensive table of NPs and their subcellular locations, will guide the future development of NP drug delivery systems for rapid expansion of biomedical applications, including cancer therapy, cardiovascular imaging, and gene therapy. PUBLIC HEALTH RELEVANCE: What this project seeks to deliver is a multi-dimensional reference table that relates the subcellular distribution and toxicity of NPs to their intrinsic physiochemical properties across a range of diverse cells and cell lines. It is our hope that the data generated from this project will serve as a resource for future research and encourage model development and new insights into nanotechnologies for imaging and drug delivery.