Tissue Resources and Molecular Pathology

病理学和组织资源核心将为实现孢子项目的目标提供常规和创新的组织资源和材料。常规材料包括肿瘤和非恶性肺标本以及肿瘤细胞系。已有3000多个肿瘤和300多个肺癌细胞系被储存起来，并向研究人员分发了5万多份肿瘤或细胞系颗粒、RNA或DNA或石蜡切片。我们的目标1是收集、处理、储存、分类和分发组织、细胞系和血液标本，包括恶性和非恶性，以及相关的临床病理和分子数据，根据孢子计划的各个组成项目的要求。目标2是开发和利用创新和常规的组织和细胞系资源，这将有助于成功完成孢子计划的目标。其中包括开发新的肿瘤细胞系资源，以补充最近关于肺癌癌基因型的全基因组突变数据。目标3是与孢子计划的组成项目密切合作，执行和解释基于组织的分子方法，以满足其批准的目标。这将涉及开发分子分析，最终可以部署为CLIA认证的实验室测试，以促进大多数SPORE项目中计划的转化科学临床试验。这个核心将在促进我们自己的孢子研究人员之间的合作方面发挥关键作用，其他肺癌孢子研究地点的研究人员，以及我们自己和其他机构的研究人员，包括由NCI/NIH和其他机构资助的其他同行评审项目。我们所有的《孢子》项目都会使用Core B的材料和服务。大量使用我们的常规和创新材料，并与SPORE研究人员密切互动，将极大地帮助我们成功完成SPORE提案的目标。

The Pathology and Tissue Resources Core will provide routine and innovative tissue resources and materials essential for achieving the aims of the SPORE projects. Routine materials include tumors and non-malignant lung specimens and tumor cell lines. Over 3,000 tumors and 300 lung cancer cell lines have been banked, and over 50,000 aliqouts of tumor or cell line pellets, RNA or DNA or paraffin sections have been distributed to investigators. Our Aim 1 is to collect, process, store, catalog and distribute tissues, cell lines and blood specimens, both malignant and nonmalignant, and relevant clinico-pathologic and molecular data, as requested by the various component projects of the SPORE program. Aim 2 is to develop and utilize innovative and routine tissue and cell line resources that will aid in the successful completion of the SPORE program aims. These include development of new tumor cell line resources to complement recent genome wide mutation data on lung cancer oncogenotypes. Aim 3 is to perform and interpret tissue-based molecular methodologies in close collaboration with the component projects of the SPORE program to satisfy their approved aims. This will involve developing molecular assays that eventually can be deployed as CLIA certified lab tests to facilitate the planned translational science clinical trials in most of the SPORE projects. This Core will play a crucial role on promoting collaboration among our own SPORE investigators, investigators at other Lung Cancer SPORE sites, and investigators at our own and other institutions including other peer-reviewed projects funded by NCI/NIH and other agencies. All of our SPORE projects will utilize Core B materials and services. Heavy utilization of our routine and innovative materials, and close interactions with the SPORE investigators will greatly aid the successful completion of the aims of our SPORE proposal.