Ultra low temperature freezer

随着人们对禽流感暴发导致另一场人类大流行的再度担忧，了解禽流感病毒如何与宿主相互作用、传播以及它们可能发生的变化变得越来越重要。从禽类的角度来看，同样重要的是开发有助于减少包括流感病毒在内的病毒疾病的发病率和传播的程序、疫苗和诊断试剂。我的研究工作是关于一组被称为禽腺病毒的病毒。这组中的一些腺病毒会引起鸡的疾病，而其他的，比如我工作的那个，是没有症状的，对鸡的健康没有影响。我选择使用的病毒被称为禽腺病毒9(FAdV-9)，它不会引起疾病，但可能是产生针对其他禽类微生物传染病(如流感)的疫苗的极佳载体。作为这项研究的一部分，我产生了FAdV-9的许多变种，以测试将这种病毒用作预防各种传染病的疫苗的可能性。为了产生这些变异，在将一些基因插入我的重组FAdV-9疫苗之前，我需要从各种微生物中分离和克隆不同的基因(通常在细菌中)。所有这些都需要一些温度敏感的分子试剂，如酶。此外，在最初在鸡身上测试各种改装的FAdV-9疫苗时，我收集了许多样本(例如血液、组织和粪便样本)，以测试病毒的存在和研究免疫反应。至关重要的是，所有这些病毒变异体、克隆中间体、分子试剂和动物组织都要在极低的温度下储存，以便为未来的分析保存它们的活性。在许多情况下，重新研究一些材料是很重要的，为此，长期、超低温储存是保存样品的最佳方式。因此，我要求提供资金，以建立一个温度足够低的超低温冷冻机，以确保长期储存，并有足够的大小来存储我在禽流感病毒和疫苗研究过程中使用和需要的许多生物样本。

With the renewed fears of an avian influenza outbreak resulting in another human pandemic, it is becoming increasingly important to understand how avian viruses interact with their host, are transmitted and how they might change. Equally important, from the avian point of view, is to develop procedures and vaccines and diagnostic reagents which can help reduce the incidence and spread of viral diseases, including influenza viruses. My research work is on a group of viruses known as avian adenoviruses. Some adenoviruses within this group cause disease in chicken, while other, like the one I work with, are asymptomatic with no effect on chicken health. The virus I chose to work with is called fowl adenovirus 9 (FAdV-9) which does not cause disease but could be an excellent vector to generate vaccines against other avian microbial infectious diseases like influenza. As part of this research, I generate numerous variants of FAdV-9 to test the possibility of using this virus as a vaccine against a variety of infectious diseases. In order to generate these variants, I need to isolate and clone (usually in bacteria) different genes from a variety of microbes before inserting some into my recombinant FAdV-9 vaccine. All of these also require a number of temperature sensitive molecular reagents such as enzymes. Moreover, while testing the various modified FAdV-9 vaccines initially in chickens, I collect many samples (e.g. blood and tissue and fecal samples) to test for the presence of the virus and to study the immune response. It is critical that all these virus variants, cloning intermediates, molecular reagents and animal tissues be stored at very low temperature to preserve their activity for future analysis. In many cases it is important to restudy some material and for that a long term, ultra cold, storage is the best way to preserve the samples. I am therefore requesting funds for an ultra low temperature freezer with a low enough temperature to ensure long term storage and of sufficient size to store the many biological samples I work with and need throughout my research on avian viruses and vaccines.