Upgrade of the confocal microscope for Concordia University

康科迪亚大学的研究人员依靠单一的共焦显微镜对生物样本进行高分辨率显微镜观察。这台显微镜是2000年2月购买的徕卡TCS SP2，不能处理一些常用的荧光团，如野生型绿色荧光蛋白和增强型黄色荧光蛋白。在目前的配置下，这种显微镜不能满足教职员工的需要，特别是对三色或更多颜色的多荧光团进行标记，因此大大降低了生产率。最后，控制系统的过时的CPU已经成为大多数基本数据处理应用的瓶颈，特别是在三维重建、动画和体重建方面。拟议的升级将允许进行更多目前并不实用的细胞生物学实验，并将加强大学的整体基础设施，促进现有教师的需求，并使该大学成为未来招聘世界级教师的更具吸引力的选择。

Researchers at Concordia University rely upon a single confocal microscope for high resolution microscopy of biological samples. This microscope, a Leica TCS SP2 purchased in February 2000, cannot handle some commonly used fluorophores such as wild type green fluorescent protein and enhanced yellow fluorescent protein. In its present configuration this microscope cannot meet the needs of the faculty, especially for multifluorophore labeling with three colours or more, and is thus greatly slowing down productivity. Finally, the outdated CPU controlling the system has become a bottleneck for most basic data processing applications, particulary for 3-D reconstruction, animation and volume reconstruction. The proposed upgrades will allow for additional cell biological experiments, not currently practical, to be performed and will enhance the overall infrastructure at the university, facilitating the needs of the current faculty and making the university a more attractive option for recruiting world-class faculty in the future.