An electrochemical strategy for high-throughput DNA Barcoding

科学名称为生物多样性提供了基本的词汇和信息框架，尽管在处理幼虫、未成熟或其他零碎的生物遗骸时，它们的应用是困难的。鉴于气候变化和日益全球化的贸易，经济发展导致物种灭绝率上升以及入侵物种和有害物种的引入，这并非微不足道。因此，地方、国家和国际社会比以往任何时候都需要更多和更快的物种鉴定服务。一项被称为DNA条形码的加拿大科学创新使用了线粒体5'端的648个碱基对序列！细胞色素c氧化酶亚基1 （CO1）基因作为动物鉴定标准。该基因靶点两侧是高度保守的区域，允许构建“通用”PCR引物，以促进该方法的广泛分类应用。然而，在某些情况下，研究人员遇到了分类群特有的普遍引物失效模式，这使得参考序列库的构建变得复杂。这是有问题的，因为一个全面的DNA序列文库是必不可少的DNA条形码鉴定。

Scientific names provide the basic vocabulary and information framework for biodiversity, though their application is difficult when dealing with larval, immature or otherwise fragmentary biological remains. This is not trivial in light of climate change and the increasing globalization of trade, as economic development leads to higher extinction rates and the introduction of invasive and pest species. As a result, local, national and international communities are demanding more and faster species identification services than ever before. A Canadian scientific innovation known as DNA barcoding uses a 648 base-pair sequence at the 5' end of the mitochondria! cytochrome c oxidase subunit 1 (CO1) gene as a standard for animal identification. This gene target is flanked by highly conserved regions that permit the construction of "universal" PCR primers needed to facilitate broad taxonomic application of the method. However, in some cases, researchers have encountered taxon specific patterns of universal primer failure that complicates the construction of the reference sequence library. This is problematic because a comprehensive DNA sequence library is essential for identification with DNA barcodes.