An electrochemical strategy for high-throughput DNA Barcoding

学名为生物多样性提供了基本的词汇和信息框架，尽管在处理幼虫、未成熟或其他支离破碎的生物遗骸时，它们的应用很困难。鉴于气候变化和贸易日益全球化，这并不是一件微不足道的事情，因为经济发展导致更高的灭绝率以及入侵和害虫物种的引入。因此，地方、国家和国际社会比以往任何时候都需要更多和更快的物种鉴定服务。加拿大的一项名为DNA条形码的科学创新在线粒体的5‘端使用了648个碱基对序列！以细胞色素c氧化酶亚基1(CO1)基因作为动物鉴定的标准。这个基因靶标的两侧是高度保守的区域，这些区域允许构建促进该方法的广泛分类学应用所需的“通用”聚合酶链式反应引物。然而，在某些情况下，研究人员遇到了通用引物失效的分类群特定模式，这使得参考序列文库的构建变得复杂。这是有问题的，因为一个全面的DNA序列文库对于使用DNA条形码进行识别是必不可少的。

Scientific names provide the basic vocabulary and information framework for biodiversity, though their application is difficult when dealing with larval, immature or otherwise fragmentary biological remains. This is not trivial in light of climate change and the increasing globalization of trade, as economic development leads to higher extinction rates and the introduction of invasive and pest species. As a result, local, national and international communities are demanding more and faster species identification services than ever before. A Canadian scientific innovation known as DNA barcoding uses a 648 base-pair sequence at the 5' end of the mitochondria! cytochrome c oxidase subunit 1 (CO1) gene as a standard for animal identification. This gene target is flanked by highly conserved regions that permit the construction of "universal" PCR primers needed to facilitate broad taxonomic application of the method. However, in some cases, researchers have encountered taxon specific patterns of universal primer failure that complicates the construction of the reference sequence library. This is problematic because a comprehensive DNA sequence library is essential for identification with DNA barcodes.