Snap Chip: portable system for miniaturized, multiplexed immunoassays

免疫测定法广泛用于研究和诊断，以定量各种样品中的蛋白质。随着我们在分子水平上对生物学和疾病的理解的进步，对能够一次测量多个参数的多路分子测试的需求越来越大。虽然DNA微阵列和其他技术允许先进的多路分析，但由于交叉反应性，抗体微阵列或头分析形式的多路免疫分析仍然昂贵且不可靠。我们已经确定了多路免疫分析缺乏可靠性的主要原因，并提出了一种新的技术和分析方案来克服这些问题；然而，所需的微阵列定位机器是昂贵的，需要在分析过程中操作，这是繁琐的，需要大量的准备。

Immunoassays are widely used in research and in diagnostics to quantify proteins in various samples. As our understanding of biology and disease at the molecular level progresses, there is a growing demand for multiplexed molecular tests that can measure multiple parameters at once. Whereas for DNA microarrays and other technologies allow for advanced multiplexed analysis, multiplexed immunoassays in the form of antibody microarrays or bead assays remain expensive and unreliable due to cross-reactivity. We had identified a major reason for the lack of reliability of multiplexed immunoassay and proposed a novel technology and assay protocol that overcomes these issues; however the microarray spotting machinery required is expensive and needs to be operated during the assay, which is cumbersome and entails significant preparation.