Liquid chromatography for ultra-high performance detection of glutathione and phytochelatins involved in the sequestration and detoxification of pollutants

本研究计划将利用超高效液相色谱法研究植物螯合素、谷胱甘肽及相关巯基肽在污染物细胞内的螯合和解毒机制。该研究仪器由核心系统、自动进样系统和荧光检测器组成，核心系统可通过专用色谱柱高效分离这些蛋白质，自动进样系统可进行高通量分析，荧光检测器可对这些蛋白质进行高灵敏度的定性和定量检测。这种分析方法将用于绿色藻类菌株暴露于不同浓度的水生污染物，以确定不同的生化特性的藻类有关的运输和封存的水生污染物在细胞液泡。通过这种方法获得的结果将被用来开发一个基本的模型，确定最好的藻类菌株特性，以获得最有效的空泡隔离污染物。该模型将用于开发新的生物技术方法来修复受污染的废水。这种利用藻类解毒机制的环境生物技术可以应用于防止水生态系统的污染和保护环境质量。

In this research program, phytochelatins, glutathione and related thiol-peptides involved in pollutants cellular sequestration and detoxification mechanisms will be investigated with ultra-high-performance liquid chromatography. This research instrument is composed of a core system permitting high-efficient separation of these proteins through specialized columns, an automated sample injection for high-throughput analysis, and a fluorescence detector providing high-sensitive qualitative and quantitative detection of these proteins. This analytical approach will be used on green algae strains exposed to different concentrations of aquatic pollutants to identify the different biochemical properties of algae related to the transport and sequestration of aquatic pollutants in the cell vacuole. The results obtained by this approach will be used to develop a fundamental model which identifies the best algal strain characteristics to obtain the most efficient vacuole sequestration of pollutants. This model will be used to develop new biotechnological approaches to remediate contaminated wastewater. This environmental biotechnology using algal detoxification mechanisms can be applied to prevent the pollution of aquatic ecosystems and protect the quality of the environment.