Fluorescence Plate Reader for Chemical Biology

荧光是一种基本现象，它提供了有关蛋白质、DNA和碳水化合物等生物大分子相互作用和功能的关键信息。因此，我们的三个生物化学小组获得荧光平板读取器对于保持我们的高水平生产力至关重要。尼茨实验室依靠这一基础设施来解开蛋白质-碳水化合物的结合作用，这是通过监测环境敏感的荧光团的排放变化来实现的。Zamble实验室依靠荧光平板阅读器来研究细菌用来调节细胞过渡金属通量和金属蛋白表达的机制。这一关键过程是由金属依赖的转录因子介导的，并通过荧光偏振实验评估了这些DNA结合蛋白的活性。Woolley实验室对光控制蛋白质的研究在很大程度上依赖于一系列荧光测量(偏振、能量转移、猝灭)来监测蛋白质在光驱动下的构象变化。荧光板阅读器的广泛应用表明了这种仪器的多功能性及其在我们的分析技术体系中的中心地位

Fluorescence is a fundamental phenomenon that provides critical information about the interactions and functions of biological macromolecules such as proteins, DNA and carbohydrates. As such, access to a fluorescence plate reader by our three biological chemistry groups is essential to maintaining our high level of productivity. The Nitz lab depends on this infrastructure to deconvolute protein-carbohydrate binding interactions, which is achieved by monitoring emission changes in environmentally sensitive fluorophores. The Zamble lab depends on the fluorescence plate reader to study the mechanisms that bacteria use to regulate cellular transition metal flux and metalloprotein expression. This key process is mediated by metal-dependent transcription factors, and the activities of these DNA-binding proteins are evaluated by fluorescence polarization experiments. The research of the Woolley lab on photo-controlled proteins relies heavily on a range of fluorescence measurements (polarization, energy transfer, quenching) for monitoring light-driven conformational changes in proteins. The breadth of these applications of the fluorescent plate reader speaks to the versatility of this piece of instrumentation and its central position in our repertoire of analytical techniques