Multimode plate reader to support research in plant biochemistry, cell biology and ecology

请拨款购买多模式车牌阅读器。所要求的仪器将用于进行各种实验，以支持植物生物化学，细胞生物学和生态学的研究项目。它是专为高通量处理，分析和定量的生物产品在微体积的样品。该仪器是多功能的，设计用于分析微孔板格式的样品，利用吸光度，荧光和发光。它还配备了一个细胞仪，允许在微孔板格式执行基本的显微镜和细胞成像。该仪器的要求配置还包括墨盒附加组件，通过允许执行时间分辨荧光分析，如荧光共振能量转移、生物发光共振能量转移和荧光偏振检测，例如在蛋白质之间相互作用的研究中，增加了仪器的灵活性和分析能力。所要求的仪器配置还能够使用铕标记的二抗定量膜上的少量蛋白质。这一特性在复杂提取物中蛋白质的定量或蛋白质修饰的研究中是有用的。由于其多模式设计，所要求的车牌读取器将取代两个不再起作用且维修费用过高的车牌读取器。它还将替换我们单位丢失的一些显微镜设备，因为最近一位研究人员去了另一所大学。它将给我们的研究所带来新的分析能力，这是开发新的研究项目所需要的。所要求的设备将集成在我们研究所的蛋白质组学和生物化学平台上，该平台以用户付费的方式运作。它将由一名合格的研究助理负责，他将负责新用户的培训和仪器的定期维护。多模式车牌阅读器将被所有四个共同申请者的实验室成员大量使用，因为他们的研究计划的许多方面依赖于一个功能的车牌阅读器的可用性。它还将为我们研究所从事分子和细胞生物学以及植物化学领域工作的其他小组的学生和研究人员提供额外的资源。根据平台的收费结构，其他学术或行业研究人员也可以使用该设备。经过此仪器培训的高素质人员将获得宝贵的实践专业知识，使用最先进的仪器，可用于就业市场上的各种基础和应用研究项目。

Funds are requested for the purchase of a multi-mode plate reader. The requested instrument will be used to perform a variety of experiments to support research programs in plant biochemistry, cell biology and ecology. It is designed for high-throughput processing, analysis and quantification of bio-products in micro-volumes of samples. This instrument is multifunctional and designed to analyze samples in microplate format using absorbance, fluorescence and luminescence. It is also equipped with a cytometer that allows to perform basic microscopy and cellular imaging in microplate format. The requested configuration of the instrument also includes cartridges add-ons that expend the flexibility and analytical capabilities of the instrument by allowing to perform time-resolved fluorescence assays for applications like Fluorescence Resonance Energy Transfer, Bioluminescence Resonance Energy Transfer and Fluorescence Polarization detection used for example in the study of interactions between proteins. The requested instrument configuration is also capable of quantifying small amounts of proteins on membranes using Europium-labeled secondary antibodies. This feature is useful in the quantification of proteins in complex extracts or in the study of protein modifications. Because of its multimode design, the requested plate reader will replace two plate readers that are no longer functional and too costly to repair. It will also replace some of the microscopy equipment lost by our unit due to the recent departure of a researcher to another university. It will bring to our Institute new analytical capabilities needed for the development of new research projects. The requested equipment will be integrated in the Proteomics and Biochemistry Platform of our Institute which functions on a user-fee basis. It will be under the responsibility of a qualified research assistant who will be in charge of the training of new users and the regular maintenance of the instrument. The multimode plate reader will be heavily used by the laboratory members of all four co-applicants because many aspects of their research programs depend on the availability of a functioning plate reader. It will also serve as an additional resource for the students and research personnel of other groups in our Institute who work in the areas of molecular and cellular biology and in phytochemistry. Other academic or industry researchers will also have access to the equipment according to the fee structure of the platform. Highly Qualified Personnel trained with this instrument will gain valuable hands-on expertise with a state of the art apparatus that can be used in a variety of fundamental and applied research projects on the job market.