Characterization of the microbial diversity found under cannabis production conditions

Lum Ultra是一家成立于北卡罗来纳州弗雷德里克顿的私营公司，开发并将微生物检测和表征技术**推向市场。为了进一步使他们的产品线多样化，**Lum Ultra请求我帮助开发基于DNA的新型试剂盒和技术，旨在检测和**量化大麻行业感兴趣的微生物。大麻产业目前在**加拿大蓬勃发展，有执照的生产商正在寻求外部专业知识来检测和量化感兴趣的微生物**(微生物植物病原体、人类病原体、有益植物有益微生物等)。目前处于**商业大麻生产条件下。因此，在该项目中，我提议与我的**合作伙伴LLumUltra合作：1)确定大麻根际和叶圈微生物群的特征；以及2)根据目标1中获得的结果，**确定目标微生物，以开发在商业大麻生产条件下使用的具体**监测定量生物测定方法。该项目将**帮助更好地了解大麻植物的微生物组，并为开发新的定量监测技术**确定微生物目标。在**商业大麻生产条件下跟踪其中一些关键微生物将对提高植物生产力、**确保适当的植物病害控制措施、以及防止人类病原体疾病暴发**以及确保大麻产品的最佳质量控制产生积极影响。

LuminUltra, a privately owned company established in Fredericton, NB, develops and brings to market**microbial detection and characterization technologies. In an effort to further diversify their line of products,**LuminUltra has requested my help to develp novel DNA-based kits and technologies aiming at detecting and**quantifying microorganisms of interest to the Cannabis industry. The cannabis industry is presently booming in**Canada and licensed producers are seeking external expertise to detect and quantify microorganisms of interest**(microbial plant pathogens, human pathogens, beneficial plant-beneficial microorganisms, etc.) present under**commercial cannabis production conditions. In this project, I therefore propose, in collaboration with my**partner LuminUltra, to: 1) characterize the cannabis rhizosphere and phyllosphere microbiomes; and 2)**identify, based on the results obtained in objective 1, target microorganisms for the development of specific**monitoring quantitative bioassays for use under commercial cannabis production conditions. This project will**help better understand the microbiome of the cannabis plant, as well as identify microbial targets for the**development of novel quantitative monitoring technologies. Tracking some of these key microorganisms under**commercial cannabis production conditions will have a positive impact on improving plant productivity,**ensuring appropriate plant disease control measures, as well as preventing human pathogen disease outbreaks**and ensuring optimal quality control of cannabis products.