Bactericidal components and mechanisms of an advanced oxidation system for water purification

埃德蒙顿的Biolargo水务公司提供了其专利高级氧化系统(AOS)消除水中细菌的概念证据。因此，该系统有可能为水消毒和去除水中的有机化合物提供一种经济上有利的替代方案。然而，该系统的杀菌作用机制仍不清楚。与目前的水消毒和净化方法不同，目前的水消毒和净化方法主要基于紫外线、氯或碘造成的氧化应激，而Biolargo的AOS快速杀灭微生物的能力是基于活性碘物种诱导的氧化应激、酸碱应激和碘施加的应激的组合。虽然细菌对这些单独应激源的耐药性是众所周知的，但几种致命或亚致命应激源联合应用对微生物生理和生存的影响尚不清楚。优化处理溶液的化学成分以及改进工艺、设备设计和扩大工艺规模需要增加对杀菌机理的了解。这项建议将通过评估选定的大肠杆菌突变体的敏感性来评估AOS的杀菌机制。在一个Engage项目中的探索性实验证明了这种实验方法产生了相关知识，并另外表明，处理液相对于有机化合物、离子和氯的组成是AOS杀菌效果的一个重要参数。该项目将对AOS产生的氧化离子物种和杀菌机制进行全面评估。在这个项目中产生的知识将被支助组织用于改进设备和工艺设计以及应用程序开发。

Biolargo Water Inc. of Edmonton has provided proof of concept that its patented Advanced Oxidation System (AOS) eliminates bacteria in water. The system thus has the potential to provide an economically advantageous alternative to water disinfection, and for removal of organic compounds in water. However, the mechanisms of the bactericidal effect of the system remain unknown. Different from current methods for water disinfection and purification, which are mainly based on oxidative stress imposed by UV light, chlorine, or iodine, Biolargo's AOS ability to rapidly kill microorganisms is based on a combination of oxidative stress that is induced by reactive iodine species, acid and alkaline stress, and stress imposed by iodine. While bacterial resistance to these individual stressors is well understood, the effect of the combined application of several of these lethal or sublethal stressors on microbial physiology and survival is unknown. Optimization of the chemical composition of the treatment solution as well as improvement of the process, equipment design, and scale up of the process requires increased knowledge on the bactericidal mechanisms. This proposal will assess bactericidal mechanisms of the AOS by evaluating the sensitivity of selected mutants of Escherichia coli. Explorative experimentation during an ENGAGE project provided proof of concept that this experimental approach generates relevant knowledge, and additionally indicated that the composition of the treatment fluid with respect to organic compounds, ions, and chlorine is an important parameter with respect to the bactericidal effect of the AOS. This project will provide a comprehensive assessment of the oxidative ion species generated by the AOS and the bactericidal mechanisms. The knowledge generated in this project will be used by the supporting organization for improved equipment and process design, and application development.