Ultra-High Performance Liquid Chromatography as a High-Throughput Analytics Platform for Organic Chemistry

高通量实验（可以同时进行成百上千个实验）的出现改变了许多物理和生命科学学科进行研究的方式。有机合成是采用高通量方法进展缓慢的一个领域，尽管有很大的发展潜力。虽然近年来工业越来越多地使用高通量方法进行反应优化和平行合成，但学术研究大多保持迭代和线性方法：每次改变一个因素进行优化，导致在烧瓶中进行单次实验合成。持续的进步要求我们的方法和工具现代化。利用我过去在制药过程化学方面的经验，利奇集团将开发新型的高通量实验，最终将为学术和工业研究人员带来进步。***高通量工作流程最重要的部分是一个快速、可靠和用户友好的分析平台。该提案为一种能够每天进行1400次以上分析的超高性能液相色谱仪器寻求资助。自动采样使*24/7操作，自动数据处理软件整理大型*数据集。据我所知，在加拿大西部的大学里没有这样的系统。这是一个独特的培训机会：学生将成为尖端分析平台的专家，并使用现代数据科学和统计技术处理大型数据集。此外，该仪器的使用将对区域工业实验室极具吸引力，从而促进学术/行业合作。***该仪器是将于2019年1月在维多利亚大学启动的新莱奇小组项目的基础。该计划旨在根据通过高通量实验获得的数据集开发化学反应性的预测模型。这是一个竞争激烈的新领域，学术和工业实验室最近发表了备受瞩目的研究。因此，确保这一工具的安全至关重要；没有它，利奇小组的研究项目就不可能实现。该提案还得到了维多利亚大学四位高级同事的共同申请支持，他们的研究项目将通过这项技术大大加快。我设想这个仪器作为一个新兴的高通量化学中心的核心能力，为所有维多利亚大学的研究人员提供服务。

The advent of high-throughput*experimentation, where hundreds-to-thousands of experiments can be run simultaneously, has changed the way many physical and life sciences disciplines*conduct research. One area that has been slow to adopt high-throughput methods,*despite significant potential for advancement, is organic synthesis. While*industry has increasingly used high-throughput approaches for reaction*optimization and parallel synthesis in recent years, academic research has*mostly kept an iterative and often linear approach: optimization by varying one*factor at a time, leading to synthesis in single experiments performed in*flasks. Continued progress requires modernization of our methods and tools.*Leveraging my past experience in pharmaceutical process chemistry, the Leitch group*will develop new kinds of high-throughput experimentation that will ultimately*lead to progress for both academic and industrial researchers.*** The most important part of high-throughput*workflows is a rapid, reliable, and user-friendly analytical platform. This*proposal seeks funding for an Ultra High Performance Liquid Chromatography*instrument capable of more than 1,400 analyses/day. Automated sampling enables*24/7 operation, and automated data processing software collates large*data sets. To my knowledge, no system with this level of throughput exists at a Western Canadian university.*This represents a unique training opportunity: students will become*experts on a cutting-edge analytical platform, as well as handle large*data sets using modern data science and statistical techniques. In addition, access to this*instrument will be highly attractive to regional industrial labs, thus fostering academic/industry collaborations.*** This instrument is foundational to the new*Leitch group program, which will get underway at UVic in January 2019. This*program seeks to develop predictive models for chemical reactivity based on*data sets obtained via high-throughput*experimentation. This is a new and competitive field, with academic and*industrial labs recently publishing high-profile studies. It is therefore*critical to secure this instrument; without it, the Leitch group research*program would be impossible. This proposal also has co-applicant support from*four senior colleagues at UVic whose research programs will be significantly*accelerated by this technology. I envision this instrument as a core capability*in an emerging center for high-throughput chemistry, available for the benefit*of all UVic researchers.