Characterization of probiotic bacterial cells enzyme activity, investigation of their gastrointestinal stability and impact on microbiota composition and function.

益生菌是活的、活跃的微生物，当摄入一定数量时，会带来显著的健康益处。Biena是一家专门从事乳制品培养、酶和益生菌的公司。Biena已经分离出几种**天然益生菌菌株，并希望开发基于**细菌细胞酶活性的各种益生菌保健配方。具体来说，Biena正在寻找基于阿魏酸酯酶活性细菌菌株的分离和鉴定细菌细胞，以**对人类健康有益。Biena缺乏基础设施和**专业知识来表征这些细菌细胞的酶活性，并研究它们在GI**转运过程中的稳定性以及这些细菌细胞对肠道细菌种群的影响，他们求助于麦吉尔大学Prakash博士的**实验室，该实验室拥有NSERC资助的计算机控制的模拟人体动力学**胃肠道模型，这是加拿大唯一的此类模型，也是该领域的专业知识。这份NSERC Engage**Plus助学金申请是贝纳大学和麦吉尔大学之间现有NSERC Engage助学金的延伸。在这项提案中，将对益生菌细胞酶活性、胃肠道稳定性以及对微生物区系组成和功能的影响进行表征。这些调查将**提供保健食品益生菌产品的开发和合作机会。

Probiotics are living, active microorganisms that, when ingested in certain quantities, confer significant health**benefits. Biena is a company specialized in dairy cultures, enzymes and probiotics. Biena has isolated several**natural probiotic bacterial strains and would like to develop various probiotic health formulations based on**bacterial cells enzymatic activities. Specifically Biena is looking to isolate and characterize bacterial cells for**human health benefits based on Ferulic Acid Esterase active bacterial strains. Biena lacks infrastructure and**expertise to characterize these bacterial cells for their enzymatic activities and investigate their stability in GI**transit and impact of those bacterial cells on gut bacterial population for which they approached Dr. Prakash's**lab at McGill University which has NSERC funded computer-controlled simulated human dynamic**gastrointestinal model, the only model of its kind in Canada and expertise in the field. This NSERC Engage**Plus Grant application is an extension of the existing NSERC Engage Grant between Beina and McGill**University. In this proposal characterization of probiotic bacterial cells enzyme activity, gastrointestinal**stability and impact on microbiota composition and function will be carried out. These investigations will**provide development of health food probiotic products and opportunities for collaborations.