Protein and amino acid metabolism in fish

该研究计划的长期目标是更好地描述和理解水生生物如何在生化和分子水平上应对环境挑战。在大多数情况下，动物的能量是精确调节的，以确保能量供应与能量需求相匹配。这种微妙的平衡可能被一些生物和非生物参数所打破，包括温度、污染、营养等。在所有情况下，ATP形式的能量被重定向到立即的救生功能，而非必要的功能被暂停，直到有利条件恢复。现在已经很清楚，蛋白质代谢就是这样一个对细胞能量平衡极其敏感的过程。短期目标是：1)表征昼夜循环缺氧后的表型调整；2)表征能量需求升高情况下蛋白质代谢的动力学；3)通过新发现的连接单碳代谢和蛋白质代谢的信号通路，评估s-腺苷蛋氨酸在蛋白质代谢控制中的意义。拟议的工作很重要，因为它关注的是进化的两个重要驱动因素，即能源供需之间的匹配和甲基的提供。这个项目的优势在于它的原创性和潜力，在各种代谢挑战中产生关于控制蛋白质代谢机制的新的重要知识。这些知识将直接应用于各个领域，特别是水产养殖、营养和保护生理学。

The long-term goal of the proposed research program is to better characterize and understand how aquatic organisms deal with environmental challenges at the biochemical and molecular level. Animal energetics is exquisitely tuned to ensure that energy supply matches energy demand in most situations. This fine balance may be tipped by several biotic and abiotic parameters including temperature, pollution, nutrition, etc. In all cases, energy in the form of ATP is redirected to immediate life-saving functions while non-essential functions are paused until favorable conditions return. It has now become clear that protein metabolism is one such process that is extremely responsive to cellular energy balance. The short-term objectives are to i) characterize the phenotypic adjustments following diel cycling hypoxia, ii) characterize the dynamics of protein metabolism in situations where energy demand is elevated and iii) Evaluate the implication of s-adenosylmethionine in the control of protein metabolism through a newly discovered signalling pathway connecting one-carbon metabolism and protein metabolism. The proposed work is important because it focusses on the two important drivers of evolution that are the match between energy supply and demand and the provision of methyl groups. The strength of this program resides in its originality and potential to generate new important knowledge on the mechanisms controlling protein metabolism during various metabolic challenges. This knowledge will find direct applications in various fields, notably in aquaculture, nutrition and conservation physiology.