Acquisition of a 500 MHz Nuclear Magnetic Resonance Spectrometer

请求提供资金购买一台多核500兆赫核磁共振分光计，供12个研究小组在各种生化、生物和化学研究项目中共用。 这些用户代表了校园内的各个部门（生物化学和生物物理学，化学，农业化学和药学），使所需的仪器成为真正的跨学科资源。 研究组分为两组：（1）主要用户（6名），他们的研究项目需要在500 MHz光谱仪上投入仪器时间，以研究生物学感兴趣分子的溶液状态动力学、动力学和/或结构功能关系;（2）次要用户（6名），其研究项目中NMR光谱不是主要研究工具，但可用于提供其他方法不易获得的有价值数据和见解。 需要这种新仪器的项目包括：DNA结合蛋白及其与DNA相互作用的结构和动力学研究;确定DNA尿嘧啶糖基化酶、其抑制剂和酶-抑制剂复合物的溶液结构;海洋天然产物和抗生素的详细结构研究;确定高度特异性灭活剂对酶的共价修饰的特异位点;并利用氧同位素诱导的碳共振频移来研究老化蛋白质的修复程度。 本提案中要求的500 MHz仪器将补充生物化学和生物物理学、化学、农业化学和药学系的现有设备。 作为一种用于表征分子结构和动力学的物理方法，NMR光谱学特别适合于生物大分子的详细研究。 在俄勒冈州立大学的500 MHz光谱仪的可用性，以及专业知识，以培训用户在收集和分析NMR数据，代表了一个重要的资源和opport团结的研究人员追求一个令人兴奋的，国家的最先进的方法至关重要的生物物理研究。

Funds are requested for purchasing a multinuclear 500 MHz nuclear magnetic resonance spectrometer to be shared by twelve research groups in various biochemical, biological and chemical research projects. These users represent various departments on campus (Biochemistry and Biophysics, Chemistry, Agricultural Chemistry, and Pharmacy), making the requested instrument a truly interdisciplinary resource. The research groups are divided into two groups: (1) Major Users (six) who have research projects that require a commitment of instrument time on a 500 MHz spectrometer to study aspects of solution-state dynamics, kinetics and /or structure function relationships of biologically interesting molecules; (2) Minor Users (six) who have research projects in which NMR spectroscopy is not a primary research tool, but can be used to provided valuable data and insight that is not easily obtainable by other methods. The projects that require this new instrumentation include: structural and dynamics studies of DNA-binding proteins and their interactions with DNA; determining the solution structures of DNA uracil glycosylase, its inhibitor, and the enzyme- inhibitor complex; detailed structural studies of marine natural products and antibiotics; identifying specific sites of covalent modification of enzymes by highly specific inactivators; and using oxygen isotope- induced carbon resonance frequency shifts to study the extent of repair in aging proteins. The 500 MHz instrument requested in this proposal will complement the existing equipment in the Departments of Biochemistry and Biophysics, Chemistry, Agricultural Chemistry, and Pharmacy. As a physical method used to characterize molecular structure and dynamics, NMR spectroscopy is uniquely suited for the detailed study of biomacromolecules. The availability of a 500 MHz spectrometer at OSU, as well as the expertise to train users in the collection and analysis of NMR data, represents an important resource and opport unity for researchers to pursue an exciting, state-of-the-art method vital to biophysical research.