Central Protein Analysis

cmc -生物分析（科隆大学CBA）和功能蛋白质组学（鲁尔大学，波宏）将作为一个加入的z项目负责基于质谱的蛋白质分析，并在与主题相关的所有技术工作流程中提供协助。在第一个资助期间，两个小组都对相互作用努力的成功做出了实质性的贡献，并展示了互补仪器和分析策略的优势。加入z项目的服务包括自上而下的蛋白质组学（1D和2D凝胶电泳，无凝胶等电聚焦，Blue Native GE）的性能，通过互补质谱设备分析蛋白质复合物和聚集体，以及与科学小组密切互动的数据评估。除了标准的蛋白质组学服务外，z项目还建立了差异蛋白质组学技术（等压标记或无标记技术），用于对真正的斑块成分进行定量分析，并为翻译后修饰蛋白质和突变亚型（位点特异性磷酸化、糖基化或泛素化/泛素化）的精细表征提供分析支持。在加入的Zproject中，两个小组的显像设备和质谱专业知识将使其成为研究小组内的一个技术平台，具有协调的数据评估和管理。特别地，这个子项目将涉及肌病相关蛋白质聚集体或蛋白质复合物的特征，有助于阐明MFM的病理机制，以及鉴定新的诊断蛋白质生物标志物候选物。

The CMMC-Bioanalytics (CBA, University of Cologne) and the Department of Functional Proteomics (Ruhr University, Bochum) will serve as a joined Z-project responsible for mass spectrometry-based protein analysis and for the assistance in all technical workflows related to the Topics. Both groups have made substantial contributions to the success of the interactive efforts during the first funding period and have demonstrated the advantages of complementary instrumentation and analysis strategies. The service of the joined Z-project covers the performance of top-down proteomics (1D and 2D gel electrophoresis, gel-free isoelectric focussing, Blue Native GE), the analysis of protein complexes and aggregates by complementary mass spectrometric equipment and a data evaluation in close interaction with the scientific groups. Beyond standard proteomic service the Z-project has established techniques in differential proteomics (isobaric-label or label-free techniques) for the quantitative analysis of genuine plaque components and offers analytical support in the fine characterization of post-translationally modified proteins and mutant isoforms (site-specific phosphorylation, glycosylation or ubiquitination/sumoylation). The apparative equipment and mass spectrometric expertise of both groups in the joined Zproject will make it to a technical platform within the Research Group with coordinated data evaluation and administration. In particular this subproject will be involved in the characterisation of myopathy-related protein aggregates or protein complexes contributing to the elucidation of pathomechanisms underlying MFM as well as to the identification of new diagnostic protein biomarker candidates.