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Central Protein Analysis

中心蛋白质分析

基本信息

批准号：
149935690
负责人：
Professor Dr. Franz-Georg Hanisch
金额：
--
依托单位：
Abteilung Funktionelle Proteomik
依托单位国家：
德国
项目类别：
Research Units
财政年份：
2009
资助国家：
德国
起止时间：
2008-12-31 至 2015-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/149935690?language=en
关键词：
Central Protein Analysis

项目摘要

The CMMC-Bioanalytics (CBA, University of Cologne) and the Department of Functional Proteomics (Ruhr University, Bochum) will serve as a joined Z-project responsible for mass spectrometry-based protein analysis and for the assistance in all technical workflows related to the Topics. Both groups have made substantial contributions to the success of the interactive efforts during the first funding period and have demonstrated the advantages of complementary instrumentation and analysis strategies. The service of the joined Z-project covers the performance of top-down proteomics (1D and 2D gel electrophoresis, gel-free isoelectric focussing, Blue Native GE), the analysis of protein complexes and aggregates by complementary mass spectrometric equipment and a data evaluation in close interaction with the scientific groups. Beyond standard proteomic service the Z-project has established techniques in differential proteomics (isobaric-label or label-free techniques) for the quantitative analysis of genuine plaque components and offers analytical support in the fine characterization of post-translationally modified proteins and mutant isoforms (site-specific phosphorylation, glycosylation or ubiquitination/sumoylation). The apparative equipment and mass spectrometric expertise of both groups in the joined Zproject will make it to a technical platform within the Research Group with coordinated data evaluation and administration. In particular this subproject will be involved in the characterisation of myopathy-related protein aggregates or protein complexes contributing to the elucidation of pathomechanisms underlying MFM as well as to the identification of new diagnostic protein biomarker candidates.

CMMC-Bioanalytics（CBA，科隆大学）和功能蛋白质组学系（鲁尔大学，波鸿）将作为一个联合Z项目，负责基于质谱的蛋白质分析，并协助与主题相关的所有技术工作流程。这两个小组在第一个供资期间为互动努力的成功作出了重大贡献，并展示了互补的仪器和分析战略的优势。加入的Z项目的服务包括自上而下的蛋白质组学（1D和2D凝胶电泳，无凝胶等电聚焦，Blue Native GE），通过互补质谱设备分析蛋白质复合物和聚集体，以及与科学小组密切互动的数据评估。除了标准的蛋白质组学服务之外，Z项目还建立了差异蛋白质组学技术（等压标记或无标记技术），用于定量分析真正的斑块成分，并为后修饰蛋白质和突变亚型（位点特异性磷酸化，糖基化或泛素化/类小泛素化）的精细表征提供分析支持。在加入的Z项目中，两个小组的外观设备和质谱专业知识将使其成为研究小组内的一个技术平台，具有协调的数据评估和管理。特别是这个子项目将参与肌病相关蛋白质聚集体或蛋白质复合物的表征，有助于阐明MFM的病理机制以及识别新的诊断蛋白质生物标志物候选物。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

P5.53 The C224W FHL1 mutation is causing a protein aggregation disorder of muscle: Two brothers revisited

P5 53 C224W FHL1 突变导致肌肉蛋白质聚集障碍：两兄弟重温

DOI：
10.1016/j.nmd.2011.06.1082
发表时间：
2011
期刊：
Neuromuscular Disorders
影响因子：
2.8
作者：
Feldkirchner;Walter MC;Kubny C;Mueller S;Kress W;Hanisch F-G;Schoser B;Schessl J
通讯作者：
Schessl J

Novel recessive myotilin mutation causes severe myofibrillar myopathy