Central Protein Analysis

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The CMMC-Bioanalytics (CBA, University of Cologne) and the Department of Functional Proteomics (Ruhr University, Bochum) will serve as a joined Z-project responsible for mass spectrometry-based protein analysis and for the assistance in all technical workflows related to the Topics. Both groups have made substantial contributions to the success of the interactive efforts during the first funding period and have demonstrated the advantages of complementary instrumentation and analysis strategies. The service of the joined Z-project covers the performance of top-down proteomics (1D and 2D gel electrophoresis, gel-free isoelectric focussing, Blue Native GE), the analysis of protein complexes and aggregates by complementary mass spectrometric equipment and a data evaluation in close interaction with the scientific groups. Beyond standard proteomic service the Z-project has established techniques in differential proteomics (isobaric-label or label-free techniques) for the quantitative analysis of genuine plaque components and offers analytical support in the fine characterization of post-translationally modified proteins and mutant isoforms (site-specific phosphorylation, glycosylation or ubiquitination/sumoylation). The apparative equipment and mass spectrometric expertise of both groups in the joined Zproject will make it to a technical platform within the Research Group with coordinated data evaluation and administration. In particular this subproject will be involved in the characterisation of myopathy-related protein aggregates or protein complexes contributing to the elucidation of pathomechanisms underlying MFM as well as to the identification of new diagnostic protein biomarker candidates.