Central Protein Analysis

CMMC生物分析中心(CBA，科隆大学)和功能蛋白质组学系(鲁尔大学，波鸿)将作为一个联合的Z项目，负责基于质谱学的蛋白质分析，并在与主题相关的所有技术工作流程中提供协助。这两个小组在第一个供资期间为互动努力的成功作出了重大贡献，并展示了互补性工具和分析战略的优势。加入的Z-项目的服务包括自上而下的蛋白质组学性能(一维和二维凝胶电泳、无凝胶等电聚焦、Blue Native GE)、利用互补的质谱仪分析蛋白质复合体和聚集体，以及与科学小组密切互动的数据评估。除了标准的蛋白质组服务外，Z-Project还建立了差异蛋白质组学技术(等压标记或无标记技术)，用于真正的斑块成分的定量分析，并在翻译后修饰蛋白质和突变异构体(位点特定的磷酸化、糖基化或泛素化/SUMo化)的精细表征方面提供分析支持。在联合Z项目中，两个小组的表观设备和质谱学专业知识将使其成为研究组内的一个技术平台，负责协调数据评估和管理。特别是，这个子项目将参与肌病相关蛋白质聚集体或蛋白质复合体的表征，有助于阐明MFM的发病机制，以及识别新的诊断蛋白质生物标记物候选。

The CMMC-Bioanalytics (CBA, University of Cologne) and the Department of Functional Proteomics (Ruhr University, Bochum) will serve as a joined Z-project responsible for mass spectrometry-based protein analysis and for the assistance in all technical workflows related to the Topics. Both groups have made substantial contributions to the success of the interactive efforts during the first funding period and have demonstrated the advantages of complementary instrumentation and analysis strategies. The service of the joined Z-project covers the performance of top-down proteomics (1D and 2D gel electrophoresis, gel-free isoelectric focussing, Blue Native GE), the analysis of protein complexes and aggregates by complementary mass spectrometric equipment and a data evaluation in close interaction with the scientific groups. Beyond standard proteomic service the Z-project has established techniques in differential proteomics (isobaric-label or label-free techniques) for the quantitative analysis of genuine plaque components and offers analytical support in the fine characterization of post-translationally modified proteins and mutant isoforms (site-specific phosphorylation, glycosylation or ubiquitination/sumoylation). The apparative equipment and mass spectrometric expertise of both groups in the joined Zproject will make it to a technical platform within the Research Group with coordinated data evaluation and administration. In particular this subproject will be involved in the characterisation of myopathy-related protein aggregates or protein complexes contributing to the elucidation of pathomechanisms underlying MFM as well as to the identification of new diagnostic protein biomarker candidates.