SGER: The Fabrication of Biological Time Resolved Arrays of Chemical Energy Readers

0129535KolodzePI建议基于MOS型晶体管结构中的电化学场效应来评估一种新型生物化学传感器的性能。他制作了这种MOS传感器的原型，并发现它们在水溶液中对DNA分子产生非线性电流-电压响应。计算表明，该装置应该对DNA和蛋白质等生物分子的电化学势高度敏感。它应该能够对时间分辨的测量进行纳秒响应时间。传感器的工作原理是基于半导体表面场效应，其中生物化合物的电化学势(费米能级)会影响晶体管类器件中的电流。检测到的生物分子位于栅绝缘体电极的德拜屏蔽长度内。计算表明，所提出的场效应器对每平方微米的有源传感器面积的灵敏度可能小于110个分子。通过将有效面积减小到100 nm平方，在光刻能力范围内，传感器可能能够检测到一个DNA分子上的电荷。之所以处于探索性和高风险状态，是因为PI尚未对不同类型的DNA和其他生物分子的传感器响应进行比较。他还没有衡量时间分辨的反应。灵敏度尚不清楚，传感器能否区分不同浓度和不同类型的DNA也是不确定的。对于这项SGER建议，PI希望测量传感器对不同类型DNA的响应，以确定其灵敏度和选择性。测量结果将用光学荧光显微镜进行校准。如果事实证明这种生物化学传感器是一种有价值的仪器，可以产生关于生物分子的有用信息，他计划提交一份全面的研究计划的完整提案。基于这种传感器的综合提案于2000年11月提交给纳米科学NIRT计划，但没有成功。审查员指出，一个弱点是缺乏关于传感器运行的足够的化学和生物数据。在这份SGER提案中，PI申请资金支持一名学生，并旅行以获得不同DNA分子和参考溶液的传感器测量结果。这些结果将被用来证明进一步的研究和大规模的资助计划是合理的。

0129535KolodzeyThe PI proposes to evaluate the performance of a novel bio-chemical sensor based on theelectrochemical field effect in MOS-type transistor structures. He has fabricatedprototypes of this MOS sensor, and have found that they produced non-linearcurrent-voltage responses to DNA molecules in an aqueous solution. Calculations indicatethat the device should be highly sensitive to the electrochemical potential of biomolecules such as DNA and proteins. It should be capable of nanosecond response times fortime-resolved measurements. These preliminary results will be described briefly here andin more detail in the Motivation Section of this proposal.The operation of the sensor is based on the semiconductor surface field effect in MOSdevices in which the electrochemical potentials (Fermi levels) of bio-compounds influencethe electrical current flowing in a transistor-like device. The detected biomolecules arelocated within a Debye screening length of the gate insulator electrode. Calculationsindicate that the proposed field effect device may be sensitive to less than 110 moleculesper square micron of active sensor area. By reducing the active area to 100 nm square,which is within lithographic capabilities, the sensor may be able to detect the charge onone DNA molecule.The reason for the exploratory and high-risk status is that the PI has not yet performed a comparison of sensor responses with different types of DNA and other biomolecules. He has not yet measured the time resolved response. The sensitivity is unknown, and it is uncertain if the sensor can distinguish different concentrations and different types of DNA. For this SGER proposal, the PI wants to measure the sensor response with different types of DNA to determine its sensitivity and selectivity. The measurements will be calibrated with an optical fluorescence microscope. If it turns out that this bio-chemical sensor is a valuable instrument that can yield useful information on biomolecules, he plans to submit a full proposal for a comprehensive research program.A comprehensive proposal based on this sensor was submitted in November 2000 to theNanoscience NIRT program, but was unsuccessful. The reviewers stated that a weakness was the lack of sufficient chemical and biological data on sensor operation. In this SGER proposal, the PI requests the funds to support a student and travel to obtain measurements of the sensor for different DNA molecules and reference solutions. These results would be used to justify further research and large funding programs.