Deciphering small molecules as virulence factors by the bacterial sugarcane pathogen Xanthomonas albilineans (ALBILINEANS)

将小分子解读为细菌性甘蔗病原体白斑黄单胞菌 (ALBILINEANS) 的毒力因子

• 批准号: 156960850
• 负责人: Professor Dr. Roderich D. Süßmuth
• 金额: --
• 依托单位: Centre de coopération internationale en recherche agronomique pour le développement (CIRAD)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/156960850?language=en
• 关键词: Deciphering; small; molecules; virulence; factors

In order to successfully colonize plants, plant pathogenic bacteria have evolved a variety of virulence factors to subvert host defences or to obtain nutrients. The understanding of the pathogenicity of these processes is of particular importance for all crop plants, since they cause enormous economic losses. Both fungal and bacterial pathogens produce secondary metabolites that play a role in plant disease. Xanthomonas albilineans (Xa) is a systemic, xylem-invading pathogen that causes leaf scald disease of sugarcane (Saccharum interspecific hybrids). The Xa-sugarcane pathosystem is an original model in plant pathology because: (i) Xa is constrained within the xylem, but disease symptoms result from changes in chlorenchyma cells; (ii) the Xa infection can be latent for many weeks or months before occurrence of acute disease; (iii) Xa produces albicidin, a unique and specific toxin that causes foliar leaf scald symptoms. Albicidin is a gyrase inhibitor and might constitute a lead structure for a novel and potent antibiotic. The genome of Xa contains 12 large unique genes with a modular architecture characteristic of NRPSs clustered in four genomic regions covering 4% of the whole genome. One of these genomic regions corresponds to previously identified albicidin biosynthesis gene cluster. In addition, our recent functional analyses strongly suggest the three other NRPS genomic regions also being involved in the pathogenicity of X. albilineans.

为了成功地定植植物，植物的致病细菌已经进化了多种毒力因子，以颠覆宿主防御或获得营养。对这些过程的致病性的理解对于所有农作物植物造成了巨大的经济损失，因此尤其重要。真菌和细菌病原体都产生次生代谢产物，在植物性疾病中起作用。 Xanthomonas albilineans（XA）是一种全身性，木质部摄取的病原体，可引起甘蔗叶（Saccharum saccharum种间杂种）的叶片炎。 Xa-甘甘烷病原系统是植物病理学中的原始模型，因为：（i）Xa在木质部内受到限制，但疾病症状是由组织细胞的变化引起的； （ii）Xa感染可能在发生急性疾病之前的多周或几个月； （iii）Xa产生阿昔丁，这是一种独特而特异的毒素，会导致叶面叶片鳞片症状。阿昔丁素是一种回旋酶抑制剂，可能构成一种新型且有效的抗生素的铅结构。 XA的基因组包含12个大型独特基因，其NRPS的模块化结构特征聚集在四个基因组区域，占整个基因组的4％。这些基因组区域之一对应于先前鉴定出的白念珠菌生物合成基因簇。此外，我们最近的功能分析强烈表明，其他三个NRP基因组区域也参与了X. albilineans的致病性。