Deciphering small molecules as virulence factors by the bacterial sugarcane pathogen Xanthomonas albilineans (ALBILINEANS)

将小分子解读为细菌性甘蔗病原体白斑黄单胞菌 (ALBILINEANS) 的毒力因子

• 批准号: 156960850
• 负责人: Professor Dr. Roderich D. Süßmuth
• 金额: --
• 依托单位: Centre de coopération internationale en recherche agronomique pour le développement (CIRAD)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/156960850?language=en
• 关键词: Deciphering; small; molecules; virulence; factors

In order to successfully colonize plants, plant pathogenic bacteria have evolved a variety of virulence factors to subvert host defences or to obtain nutrients. The understanding of the pathogenicity of these processes is of particular importance for all crop plants, since they cause enormous economic losses. Both fungal and bacterial pathogens produce secondary metabolites that play a role in plant disease. Xanthomonas albilineans (Xa) is a systemic, xylem-invading pathogen that causes leaf scald disease of sugarcane (Saccharum interspecific hybrids). The Xa-sugarcane pathosystem is an original model in plant pathology because: (i) Xa is constrained within the xylem, but disease symptoms result from changes in chlorenchyma cells; (ii) the Xa infection can be latent for many weeks or months before occurrence of acute disease; (iii) Xa produces albicidin, a unique and specific toxin that causes foliar leaf scald symptoms. Albicidin is a gyrase inhibitor and might constitute a lead structure for a novel and potent antibiotic. The genome of Xa contains 12 large unique genes with a modular architecture characteristic of NRPSs clustered in four genomic regions covering 4% of the whole genome. One of these genomic regions corresponds to previously identified albicidin biosynthesis gene cluster. In addition, our recent functional analyses strongly suggest the three other NRPS genomic regions also being involved in the pathogenicity of X. albilineans.

植物病原细菌为了在植物上成功定殖，进化出了多种毒力因子，以破坏寄主的防御或获取营养。了解这些过程的致病性对所有作物都特别重要，因为它们会造成巨大的经济损失。真菌和细菌病原体都产生在植物病害中起作用的次级代谢产物。白纹黄单胞菌（Xanthomonasalbilineans，Xa）是甘蔗种间杂种的一种系统性木质部入侵病原菌。Xa-甘蔗病理系统是植物病理学中的原始模型，因为：（i）Xa被限制在木质部内，但疾病症状由薄壁组织细胞的变化引起;（ii）Xa感染可以在急性疾病发生之前潜伏数周或数月;（iii）Xa产生albicidin，一种独特且特异的毒素，其引起叶面叶片萎蔫症状。Albicidin是一种促旋酶抑制剂，可能构成一种新型有效抗生素的先导结构。Xa的基因组包含12个大的独特的基因，具有NRPS的模块化结构特征，聚集在4个基因组区域中，覆盖整个基因组的4%。这些基因组区域之一对应于先前鉴定的albicidin生物合成基因簇。此外，我们最近的功能分析强烈建议其他三个NRPS基因组区域也参与X的致病性。阿尔比利尼人