Deciphering small molecules as virulence factors by the bacterial sugarcane pathogen Xanthomonas albilineans

将小分子解读为细菌性甘蔗病原体白斑黄单胞菌的毒力因子

• 批准号: 236361684
• 负责人: Professor Dr. Roderich D. Süßmuth
• 金额: --
• 依托单位: Centre de coopération internationale en recherche agronomique pour le développement (CIRAD)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/236361684?language=en
• 关键词: Deciphering; small; molecules; virulence; factors

In order to successfully colonize plants, plant pathogenic bacteria have evolved a variety of virulence factors to subvert host defences or to obtain nutrients. The understanding of these pathogenic processes is of particular importance since bacteria are capable of causing enormous economic losses on crop plants. Xanthomonas albilineans (Xa) is a systemic, xylem-invading pathogen that causes leaf scald, a lethal disease of sugarcane (Saccharum interspecific hybrids). The Xa-sugarcane pathosystem is an original model in plant pathology because: (i) Xa is constrained within the xylem, but disease symptoms result from changes in chlorenchyma cells; (ii) the Xa infection can be latent for many weeks or months before occurrence of acute disease. Sequencing of the genome of Xa revealed the absence of the type III secretion system Hrp and rather suggested that pathogenicity is dependent on the biosynthesis of secondary metabolites. The most prominent representative is albicidin, a specific toxin that causes foliar leaf scald symptoms. Furthermore, genome sequencing results indicate the presence of genes encoding for additional three non-ribosomal peptide synthetase gene clusters (Metacols A, B and C). The production of secondary metabolites implies additional functions as effectors or toxins in pathogenesis of Xa. Hence, the project is directed to the isolation and structure elucidation of albicidin, its derivatives and of secondary metabolites Metacols A-C. The study will further aim at the characterization of the bioactivities of Metacols A-C to better understand how X. albilineans successfully spreads in sugarcane xylem vessels. The work will have an impact on the understanding of the Xa-sugarcane pathosystem and also two other pathosystems involving species of Xanthomonas, which possess a Metacol-B like NRPS gene cluster (the rice pathogen Xanthomonas oryzae and the wheat pathogen Xanthomonas transluscens). Finally, albicidin may server as a lead structure for novel antibiotics.

为了成功定植植物，植物病原菌进化出多种毒力因子来颠覆宿主防御或获取营养。了解这些致病过程尤为重要，因为细菌能够对农作物造成巨大的经济损失。白叶黄单胞菌 (Xa) 是一种全身性、侵入木质部的病原体，可导致叶烫伤，这是一种甘蔗（甘蔗种间杂种）的致命疾病。 Xa-甘蔗病理系统是植物病理学的原始模型，因为：（i）Xa 被限制在木质部内，但疾病症状是由绿藻细胞的变化引起的； (ii) Xa 感染可在急性疾病发生前潜伏数周或数月。 Xa 基因组测序揭示了 III 型分泌系统 Hrp 的缺失，并表明致病性取决于次级代谢产物的生物合成。最突出的代表是albicidin，一种引起叶面叶烫伤症状的特定毒素。此外，基因组测序结果表明存在编码另外三个非核糖体肽合成酶基因簇（Metacols A、B 和 C）的基因。次级代谢产物的产生意味着 Xa 发病机制中作为效应物或毒素的附加功能。因此，该项目致力于 Albicidin、其衍生物和次级代谢物 Metacols A-C 的分离和结构阐明。该研究将进一步旨在表征 Metacols A-C 的生物活性，以更好地了解 X. albilineans 如何在甘蔗木质部导管中成功传播。这项工作将对 Xa-甘蔗病理系统以及涉及黄单胞菌属物种的另外两个病理系统产生影响，这些黄单胞菌属物种具有类似 NRPS 基因簇的 Metacol-B（水稻病原体米黄单胞菌和小麦病原体透明黄单胞菌）。最后，albicidin 可以作为新型抗生素的先导结构。

项目成果

The O-Carbamoyl-Transferase Alb15 Is Responsible for the Modification of Albicidin.

O-氨基甲酰基转移酶 Alb15 负责 albicidin 的修饰

• DOI: 10.1021/acschembio.5b01001
• 发表时间: 2016
• 期刊: ACS chemical biology
• 影响因子: 4
• 作者: D. Petras;D. Kerwat;A. Pesic;B.F. Hempel;L. von Eckardstein;S. Semsary;J. Arasté;M. Marguerettaz;M. Royer;S. Cociancich;R.D. Süssmuth
• 通讯作者: R.D. Süssmuth

What makes Xanthomonas albilineans unique amongst xanthomonads?

• DOI: 10.3389/fpls.2015.00289
• 发表时间: 2015
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Pieretti I;Pesic A;Petras D;Royer M;Süssmuth RD;Cociancich S
• 通讯作者: Cociancich S

The Albicidin Resistance Factor AlbD Is a Serine Endopeptidase That Hydrolyzes Unusual Oligoaromatic-Type Peptides.

Albicidin 抗性因子 AlbD 是一种丝氨酸内肽酶，可水解不寻常的寡芳香族类型肽

• DOI: 10.1021/jacs.5b04099
• 发表时间: 2015
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: L. Vieweg;J. Kretz;A. Pesic;D. Kerwat;S. Grätz;M. Royer;S. Cociancich;A. Mainz;R.D. Süssmuth
• 通讯作者: R.D. Süssmuth