Deciphering small molecules as virulence factors by the bacterial sugarcane pathogen Xanthomonas albilineans

将小分子解读为细菌性甘蔗病原体白斑黄单胞菌的毒力因子

• 批准号: 236361684
• 负责人: Professor Dr. Roderich D. Süßmuth
• 金额: --
• 依托单位: Centre de coopération internationale en recherche agronomique pour le développement (CIRAD)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/236361684?language=en
• 关键词: Deciphering; small; molecules; virulence; factors

In order to successfully colonize plants, plant pathogenic bacteria have evolved a variety of virulence factors to subvert host defences or to obtain nutrients. The understanding of these pathogenic processes is of particular importance since bacteria are capable of causing enormous economic losses on crop plants. Xanthomonas albilineans (Xa) is a systemic, xylem-invading pathogen that causes leaf scald, a lethal disease of sugarcane (Saccharum interspecific hybrids). The Xa-sugarcane pathosystem is an original model in plant pathology because: (i) Xa is constrained within the xylem, but disease symptoms result from changes in chlorenchyma cells; (ii) the Xa infection can be latent for many weeks or months before occurrence of acute disease. Sequencing of the genome of Xa revealed the absence of the type III secretion system Hrp and rather suggested that pathogenicity is dependent on the biosynthesis of secondary metabolites. The most prominent representative is albicidin, a specific toxin that causes foliar leaf scald symptoms. Furthermore, genome sequencing results indicate the presence of genes encoding for additional three non-ribosomal peptide synthetase gene clusters (Metacols A, B and C). The production of secondary metabolites implies additional functions as effectors or toxins in pathogenesis of Xa. Hence, the project is directed to the isolation and structure elucidation of albicidin, its derivatives and of secondary metabolites Metacols A-C. The study will further aim at the characterization of the bioactivities of Metacols A-C to better understand how X. albilineans successfully spreads in sugarcane xylem vessels. The work will have an impact on the understanding of the Xa-sugarcane pathosystem and also two other pathosystems involving species of Xanthomonas, which possess a Metacol-B like NRPS gene cluster (the rice pathogen Xanthomonas oryzae and the wheat pathogen Xanthomonas transluscens). Finally, albicidin may server as a lead structure for novel antibiotics.

植物病原细菌为了在植物上成功定殖，进化出了多种毒力因子，以破坏寄主的防御或获取营养。这些致病过程的理解是特别重要的，因为细菌能够造成巨大的经济损失的作物。白纹黄单胞菌（Xanthomonasalbilineans，Xa）是一种系统性的木质部入侵病原菌，可引起甘蔗叶枯病，是甘蔗种间杂种的致死性病害。Xa-甘蔗病理系统是植物病理学中的原始模型，因为：（i）Xa被限制在木质部内，但疾病症状由薄壁组织细胞的变化引起;（ii）Xa感染可以在急性疾病发生之前潜伏数周或数月。Xa基因组的测序结果表明，缺乏III型分泌系统Hrp，而是表明，致病性是依赖于次生代谢产物的生物合成。最突出的代表是albicidin，这是一种引起叶枯症状的特异性毒素。此外，基因组测序结果表明存在编码另外三种非核糖体肽合成酶基因簇（Metacols A、B和C）的基因。次生代谢产物的产生意味着在Xa的发病机制中作为效应物或毒素的额外功能。因此，该项目旨在分离和结构阐明albicidin，其衍生物和次级代谢产物Metacols A-C。该研究将进一步旨在表征Metacols A-C的生物活性，以更好地了解X。albilineans在甘蔗木质部导管中成功传播。这项工作将对了解Xa-甘蔗病理系统以及涉及黄单胞菌属物种的另外两个病理系统产生影响，这些物种具有类似Metacol-B的NRPS基因簇（水稻病原体水稻黄单胞菌和小麦病原体黄单胞菌）。透明）。最后，albicidin可能作为新型抗生素的先导结构。

项目成果

The O-Carbamoyl-Transferase Alb15 Is Responsible for the Modification of Albicidin.

O-氨基甲酰基转移酶 Alb15 负责 albicidin 的修饰

• DOI: 10.1021/acschembio.5b01001
• 发表时间: 2016
• 期刊: ACS chemical biology
• 影响因子: 4
• 作者: D. Petras;D. Kerwat;A. Pesic;B.F. Hempel;L. von Eckardstein;S. Semsary;J. Arasté;M. Marguerettaz;M. Royer;S. Cociancich;R.D. Süssmuth
• 通讯作者: R.D. Süssmuth

What makes Xanthomonas albilineans unique amongst xanthomonads?

• DOI: 10.3389/fpls.2015.00289
• 发表时间: 2015
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Pieretti I;Pesic A;Petras D;Royer M;Süssmuth RD;Cociancich S
• 通讯作者: Cociancich S

The Albicidin Resistance Factor AlbD Is a Serine Endopeptidase That Hydrolyzes Unusual Oligoaromatic-Type Peptides.

Albicidin 抗性因子 AlbD 是一种丝氨酸内肽酶，可水解不寻常的寡芳香族类型肽

• DOI: 10.1021/jacs.5b04099
• 发表时间: 2015
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: L. Vieweg;J. Kretz;A. Pesic;D. Kerwat;S. Grätz;M. Royer;S. Cociancich;A. Mainz;R.D. Süssmuth
• 通讯作者: R.D. Süssmuth