Continuous Optical Fractionation of Biological Materials

生物材料的连续光学分离

• 批准号: 0233971
• 负责人: David Grier
• 金额: $ 55.31万
• 依托单位: University of Chicago
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0233971&HistoricalAwards=false
• 关键词: Continuous; Optical; Fractionation; Biological; Materials

This award supports development of a compact, integrated system for continuous separation of fluid-borne materials such as macromolecules, nanoclusters, colloidal particles, cell fragments and entire living cells. The device is based on optical fractionation, a recently discovered strategy based on differing interactions with light to distinguish and sort specified subpopulations of particles - for example, cells of differing size - within heterogeneous samples (complex mixtures). The device uses arrays of optical traps to selectively deflect materials into microfluidic channels for collection and processing. The basic principle has been demonstrated on the laboratory scale. The current grant will support (1) development of the technology into a less expensive and more compact, turn-key system, and (2) development of protocols for optically fractionating different classes of biomaterials. The optical fractionation instrument offers continuous sample sorting and separation with an unprecedented combination of accuracy, generality, ease of use and cost-effectiveness. Consequently, it should find widespread applications in biological research, ranging from laboratory purification of both synthetic and natural biomolecules to new modes of cell cytometry. In addition to development of the instrument, the project is expected to provide the basis for two doctoral theses and three undergraduate honors theses in biophysics.

该奖项支持开发紧凑的集成系统，用于连续分离流体传播材料，例如大分子，纳米簇，胶体颗粒，细胞碎片和整个活细胞。该设备基于光学分馏，这是一种基于与光的不同相互作用的最近发现的策略，以区分和对粒子的指定亚群（例如，在异质样品中的大小不同的细胞）（复杂的混合物）内进行了分类。该设备使用光学陷阱的数组将材料有选择地将材料转移到微流体通道中进行收集和处理。基本原理已在实验室范围内证明。 当前的赠款将支持（1）将技术开发成更便宜，更紧凑，旋转键的系统，以及（2）开发用于光学分数不同类别生物材料的协议。 光学分级仪器提供了连续样品分类和分离，具有前所未有的精度，一般性，易用性和成本效益的组合。因此，它应该在生物学研究中找到广泛的应用，从合成和天然生物分子的实验室纯化到细胞细胞仪的新模式。 除了开发仪器外，该项目有望为生物物理学的两个博士学位论文和三个本科荣誉提供基础。