How to live in a mosaic of stressors - an ecological genomics approach on the water flea Daphnia

如何生活在压力源的镶嵌体中——水蚤水蚤的生态基因组学方法

• 批准号: 166183299
• 负责人: Professor Dr. Christian Laforsch
• 金额: --
• 依托单位: Helsingin Yliopisto
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/166183299?language=en
• 关键词: How; live; mosaic; stressors; ecological

STRESSFLEA will develop and use genomics tools to unravel patterns and mechanisms of adaptation to anthropogenic and natural stressors in natural populations, using the water flea Daphnia magna as a model system. Daphnia is a key model organism in ecology, evolutionary biology and ecotoxicology, and rapidly develops to become a leading model invertebrate in ecological genomics. Daphnia has clear assets as an ecogenomic model because of its ecological importance and life cycle features (short generation time, clonal lineages, layered dormant egg banks). STRESSFLEA aims to (1) obtain insight into the genomic underpinning of genetic adaptation to specific stressors (predation, parasitism, habitat unpredictability, metal pollution); (2) identify gene function by linking gene expression to trait values; (3) obtain insight into the genomics of adaptation to multiple stressors; and (4) reconstruct evolutionary processes over an extended time axis through the use of genomic markers and candidate genes in layered egg banks. STRESSFLEA brings together the key European research groups developing genomic resources for studying responses to stressors in natural Daphnia populations (supplemented with a pivotal research group from the US), and combines transcriptome analysis, targeted genome scans, QTL analysis and gene mapping, microarrays, proteomics and methylome analysis to identify candidate loci and associated SNP markers, analyse gene function, and apply this knowledge to reconstruct evolutionary dynamics in a paleogenomics approach and analyse the dynamics and signature of local adaptation. The legacy of STRESSFLEA will include firm insight into the genomic underpinning of local adaptation to single and multiple stressors, insight into the dynamics of micro-evolutionary adaptation over extended timescales, a strongly elaborated genomics and transcriptomics toolbox for ecologic genomics using the model system Daphnia, and a firmly established network of European research groups that collaborate on ecological and functional genomics of natural populations, using Daphnia as a model.

stress - flea将开发并使用基因组学工具来揭示自然种群对人为和自然压力源的适应模式和机制，以大水蚤为模型系统。水蚤是生态学、进化生物学和生态毒理学的重要模式生物，并迅速发展成为生态基因组学中领先的模式无脊椎动物。水蚤具有重要的生态学意义和生命周期特征（世代时间短、无性系、分层休眠卵库），因此作为一种生态基因组模型具有明显的优势。stress - flea旨在(1)深入了解基因适应特定应激源（捕食、寄生、栖息地不可预测性、金属污染）的基因组基础；(2)通过将基因表达与性状值联系起来，鉴定基因功能；(3)深入了解多种应激源适应的基因组学；(4)通过使用基因组标记和层状卵子库中的候选基因，在延长的时间轴上重建进化过程。stress - flea汇集了欧洲主要研究团队开发的基因组资源，用于研究天然水蚤种群对压力源的反应（辅以来自美国的关键研究团队），并结合转录组分析，靶向基因组扫描，QTL分析和基因定位，微阵列，蛋白质组学和甲基化分析，以确定候选位点和相关SNP标记，分析基因功能，并将这些知识应用于古基因组学方法中重建进化动力学，并分析局部适应的动力学和特征。stress - flea的遗产将包括：深入了解局部适应单一和多重压力源的基因组基础，深入了解延长时间尺度上微观进化适应的动态，使用水蚤模型系统的生态基因组学和转录组学工具箱，以及牢固建立的欧洲研究小组网络，合作研究自然种群的生态和功能基因组学，使用水蚤作为模型。