Microbial Genome Sequencing: Genome Sequence of the Model Biotroph Peronospora parasitica

微生物基因组测序：模型生物营养型霜孢菌的基因组序列

• 批准号: 0412213
• 负责人: Brett Tyler
• 金额: --
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0412213&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Sequence; Model

Obligate biotrophic plant pathogens exhibit a highly evolved relationship with their hosts, being completely dependent on living host tissue for survival. Many of the most destructive plant pathogens are obligate pathogens, including the downy mildews, powdery mildews and rusts. The maize downy mildews Peronosclerospora philippinesis and Sclerophthora rayssiae and the soybean rust Phakopsora pachyrhizi are considered major crop biosecurity threats to the US. However, genetic analysis of obligate biotrophs is difficult because they cannot be grown apart from their plant hosts. As part of an international initiative a draft genome sequence will be developed for Peronospora parasitica, a downy mildew of Arabidopsis thaliana and placed into a community annotation database. UK collaborators will carry out sequencing of ESTs and fingerprinting and sequencing of BAC clones. Pe. parasitica is a compelling choice for studying obligate biotrophy. Firstly it has culturable close relatives, such as Phytophthora infestans and Phytophthora sojae, that have excellent genome resources and are amenable to genetic manipulation, facilitating functional analysis of Pe. parasitica genes. Secondly, the host of Pe. parasitica, Arabidopsis is the leading plant model system, so that there has already been extensive physiological, genetic and genomics studies of the interaction between Pe. parasitica and its host.Broader ImpactsThe project will also create a resource for the plant-microbe interaction research community that will enable significant advances in the understanding of how biotrophic pathogens attack plants, and consequently, the design of better methods for protecting crops and natural ecosystems from these pathogens. The community annotation platform and annotation jamboree will provide multi-disciplinary training environments for postdoctoral fellows, graduate students, undergraduates from all US and international institutions.

专性生物营养型植物病原体表现出与其宿主的高度进化关系，完全依赖活的宿主组织生存。许多最具破坏性的植物病原体是专性病原体，包括霜霉病、白粉病和锈病。玉米霜霉病Peronoscillospora philippinesis和Sclerophthora raysiae以及大豆锈病Phakopsora pachyrhizi被认为是美国主要的作物生物安全威胁。然而，专性生物营养体的遗传分析是困难的，因为它们不能生长远离其植物宿主。 作为一项国际倡议的一部分，将为一种拟南芥霜霉病菌Peronospora parasitica开发基因组序列草案，并将其放入社区注释数据库。英国的合作者将进行EST的测序和BAC克隆的指纹和测序。 佩寄生菌是研究专性生物营养的一个引人注目的选择。首先，它有可培养的近亲，如致病疫霉和大豆疫霉，具有优良的基因组资源，并适合遗传操作，促进Pe的功能分析。寄生虫基因。第二，主持人。拟南芥（Arabidopsis）是植物模式系统中最重要的模式植物，因此，人们已经在生理学、遗传学和基因组学等方面对Pe.更广泛的影响该项目还将为植物-微生物相互作用研究界创造一个资源，这将使人们在理解活体营养病原体如何攻击植物方面取得重大进展，从而设计出更好的方法来保护作物和自然生态系统免受这些病原体的侵害。社区注释平台和注释大会将为来自所有美国和国际机构的博士后研究员，研究生，本科生提供多学科的培训环境。