Microbial Genome Sequencing: Genome Sequence of the Model Biotroph Peronospora parasitica

专性生物营养植物病原体与其寄主表现出高度进化的关系，完全依赖于活的寄主组织生存。许多最具破坏性的植物病原体是专性病原体，包括霜霉病，白粉病和锈病。玉米霜霉病（peronossclerospora philippinesis）和疫霉（Sclerophthora rayssiae）以及大豆锈病（Phakopsora pachyrhizi）被认为是对美国农作物生物安全的主要威胁。然而，专性生物营养物的遗传分析是困难的，因为它们不能与它们的植物宿主分开生长。作为一项国际倡议的一部分，将为拟南芥的霜霉病Peronospora parasitica制定基因组序列草案，并将其放入社区注释数据库。英国合作者将对est进行测序，对BAC克隆进行指纹识别和测序。体育。寄生生物是研究专性生物营养的重要选择。首先，它具有可培养的近亲属，如疫霉菌和大豆疫霉菌，具有优良的基因组资源，易于遗传操作，便于Pe的功能分析。parasitica基因。其次，主持人的Pe。拟南芥是主要的寄生植物模式系统，因此已经有广泛的生理、遗传和基因组学研究。寄生物及其寄主。更广泛的影响该项目还将为植物-微生物相互作用研究界创造资源，这将使对生物营养病原体如何攻击植物的理解取得重大进展，从而设计出更好的方法来保护作物和自然生态系统免受这些病原体的侵害。社区标注平台和标注大会将为来自美国和国际机构的博士后、研究生、本科生提供多学科的培训环境。

Obligate biotrophic plant pathogens exhibit a highly evolved relationship with their hosts, being completely dependent on living host tissue for survival. Many of the most destructive plant pathogens are obligate pathogens, including the downy mildews, powdery mildews and rusts. The maize downy mildews Peronosclerospora philippinesis and Sclerophthora rayssiae and the soybean rust Phakopsora pachyrhizi are considered major crop biosecurity threats to the US. However, genetic analysis of obligate biotrophs is difficult because they cannot be grown apart from their plant hosts. As part of an international initiative a draft genome sequence will be developed for Peronospora parasitica, a downy mildew of Arabidopsis thaliana and placed into a community annotation database. UK collaborators will carry out sequencing of ESTs and fingerprinting and sequencing of BAC clones. Pe. parasitica is a compelling choice for studying obligate biotrophy. Firstly it has culturable close relatives, such as Phytophthora infestans and Phytophthora sojae, that have excellent genome resources and are amenable to genetic manipulation, facilitating functional analysis of Pe. parasitica genes. Secondly, the host of Pe. parasitica, Arabidopsis is the leading plant model system, so that there has already been extensive physiological, genetic and genomics studies of the interaction between Pe. parasitica and its host.Broader ImpactsThe project will also create a resource for the plant-microbe interaction research community that will enable significant advances in the understanding of how biotrophic pathogens attack plants, and consequently, the design of better methods for protecting crops and natural ecosystems from these pathogens. The community annotation platform and annotation jamboree will provide multi-disciplinary training environments for postdoctoral fellows, graduate students, undergraduates from all US and international institutions.