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Integration von Expressions- und genomweiten Assoziations-Analysen in Kreuzungspopulationen kommerzieller Rassen zur Kartierung von QTL Regionen mit verbesserter Auflösung und funktionelle Analyse von Kandidatengenen für Wasserbindungsvermögen

在商业品种的杂交群体中整合表达和全基因组关联分析，以提高分辨率和水结合能力候选基因的功能分析来绘制 QTL 区域

基本信息

批准号：
168145480
负责人：
Professor Dr. Karl Schellander
金额：
--
依托单位：
Abteilung Tierzucht und Tierhaltung
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2009
资助国家：
德国
起止时间：
2008-12-31 至 2012-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/168145480?language=en
关键词：
Integration von Expressions und genomweiten

项目摘要

Variability in meat quality traits like water holding capacity (WHC) is a major concern for producers and consumers. In the first phase of `DRIP´ we have already shown that many biological pathways including extracellular matrix receptor interaction, calcium signalling, oxidative phosphorylation, mitochondrial pathways, actin cytoskeleton signalling, PDGF signalling, EGF signalling, ERK/MAPK signalling, as well as TR/RXR activation are correlated with WHC. The combination of expression data with linkage studies offers new options to understand the biology at a global level and the genetic factors affecting the trait of interest. Expression-quantitative trait loci (eQTL) studies of genes with trait-correlated expression may provide the necessary information required for identifying genes that control quantitative phenotypes. We have identified a number of cis and trans eQTL that coincide with those of the QTL for drip loss (DRIP) identified in a F2 pig population. In order to refine these results additional selected F2 animals with maximized genetic and phenotypic dissimilarity will be used for expression profiling and genotyping. To overcome the weakness of a still limited mapping resolution within the F2-Families and to validate and confirm the biological pathways commercial populations will be used to validate and refine the results. Here, a whole genome association study for the muscle gene expression profiles of the commercial herd Pietrain x (German Landrace x German Large White) (Pix(DLxDE)) will be studied and the results obtained in this population will be compared with previously linkage studies in the F2 resource population. The list of candidate genes and linkage regions confirmed in both phase I and II will be used for further functional analysis.

肉质性状的变异性，如持水力（WHC），是生产者和消费者关注的主要问题。在“DRIP”的第一阶段，我们已经表明许多生物学途径，包括细胞外基质受体相互作用，钙信号传导，氧化磷酸化，线粒体途径，肌动蛋白细胞骨架信号传导，PDGF信号传导，EGF信号传导，ERK/MAPK信号传导以及TR/RXR激活与WHC相关。表达数据与连锁研究的结合为在全球水平上理解生物学和影响感兴趣性状的遗传因素提供了新的选择。表达-数量性状基因座（expression-quantitative trait loci，eQTL）研究可为鉴定控制数量表型的基因提供必要的信息。我们已经确定了一些顺式和反式eQTL，与在F2猪群体中确定的滴水损失（DRIP）QTL相一致。为了完善这些结果，将使用具有最大遗传和表型差异的额外选定F2动物进行表达谱分析和基因分型。为了克服F2家族内仍然有限的映射分辨率的弱点，并验证和确认生物学途径，将使用商业群体来验证和完善结果。在这里，将研究商业牛群Pietrain x（德国长白牛x德国大白色）（Pix（DLxDE））肌肉基因表达谱的全基因组关联研究，并将在该群体中获得的结果与先前在F2资源群体中的连锁研究进行比较。在I期和II期确认的候选基因和连锁区域的列表将用于进一步的功能分析。