Integration von Expressions- und genomweiten Assoziations-Analysen in Kreuzungspopulationen kommerzieller Rassen zur Kartierung von QTL Regionen mit verbesserter Auflösung und funktionelle Analyse von Kandidatengenen für Wasserbindungsvermögen

肉类品质性状的变化，如持水量（WHC），是生产者和消费者关注的主要问题。在“DRIP”的第一阶段，我们已经证明了许多生物途径，包括细胞外基质受体相互作用，钙信号传导，氧化磷酸化，线粒体途径，肌动蛋白细胞骨架信号传导，PDGF信号传导，EGF信号传导，ERK/MAPK信号传导，以及TR/RXR激活与WHC相关。表达数据与连锁研究的结合为理解全球水平的生物学和影响感兴趣性状的遗传因素提供了新的选择。性状相关表达基因的表达-数量性状位点（eQTL）研究可以为鉴定控制数量表型的基因提供必要的信息。我们已经确定了一些顺式和反式QTL，这些QTL与F2猪群体中发现的滴漏损失QTL一致。为了完善这些结果，我们将使用遗传和表型差异最大的F2动物进行表达谱和基因分型。为了克服f2家族内仍然有限的制图分辨率的弱点，并验证和确认生物途径，将使用商业种群来验证和完善结果。本研究将对商业畜群Pietrain x（德国长白猪x德国大白猪）（Pix(DLxDE)）肌肉基因表达谱进行全基因组关联研究，并将在该群体中获得的结果与之前在F2资源群体中进行的连锁研究进行比较。在I期和II期确认的候选基因和连锁区域列表将用于进一步的功能分析。

Variability in meat quality traits like water holding capacity (WHC) is a major concern for producers and consumers. In the first phase of `DRIP´ we have already shown that many biological pathways including extracellular matrix receptor interaction, calcium signalling, oxidative phosphorylation, mitochondrial pathways, actin cytoskeleton signalling, PDGF signalling, EGF signalling, ERK/MAPK signalling, as well as TR/RXR activation are correlated with WHC. The combination of expression data with linkage studies offers new options to understand the biology at a global level and the genetic factors affecting the trait of interest. Expression-quantitative trait loci (eQTL) studies of genes with trait-correlated expression may provide the necessary information required for identifying genes that control quantitative phenotypes. We have identified a number of cis and trans eQTL that coincide with those of the QTL for drip loss (DRIP) identified in a F2 pig population. In order to refine these results additional selected F2 animals with maximized genetic and phenotypic dissimilarity will be used for expression profiling and genotyping. To overcome the weakness of a still limited mapping resolution within the F2-Families and to validate and confirm the biological pathways commercial populations will be used to validate and refine the results. Here, a whole genome association study for the muscle gene expression profiles of the commercial herd Pietrain x (German Landrace x German Large White) (Pix(DLxDE)) will be studied and the results obtained in this population will be compared with previously linkage studies in the F2 resource population. The list of candidate genes and linkage regions confirmed in both phase I and II will be used for further functional analysis.