A Hybrid Quadrupole Time-of-Flight Tandem Mass Spectrometer Integrated with UPLC for Characterization of Protein Complexes and Comparative Proteomics

与 UPLC 集成的混合四极杆飞行时间串联质谱仪用于蛋白质复合物的表征和比较蛋白质组学

• 批准号: 0500689
• 负责人: Klaas van Wijk
• 金额: $ 34.86万
• 依托单位: Cornell Univ - State: AWDS MADE PRIOR MAY 2010
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0500689&HistoricalAwards=false
• 关键词: Hybrid; Quadrupole; Time; Flight; Tandem

This award provides funds to aid in the purchase of a hybrid Quadrupole Time-of-Flight Tandem Mass spectrometer (Q-TOF), interfaced with a fully integrated ultrahigh pressure liquid chromatography (UPLC) system. The instrument will be central to research supported NSF research grants awarded to the six major users, including the Cornell Proteomics Core facility, and multiple minor users. The instrument will complement existing equipment in the proteomics core facility and the Department of Plant Biology, particularly through its application in the analysis of protein-protein and protein-ligand interactions and in de-novo sequencing of proteins from organisms whose genomic sequence is unknown. Highly sensitive and accurate tandem mass spectrometers are critical in the identification and characterization of low abundance proteins. In addition, the instrument will be a key tool for comparative quantitative (plant) proteomics studies using stable isotope labeling. The mass spectrometer will accelerate and facilitate a wide range of research projects of Cornell Faculty across different departments. The instrument is also expected to aid in the training of more than 40 undergraduate, graduate, and postdoctoral students, and will play a role in an NSF-funded outreach program in 'Plant Genomics', a program that attracts high school students and teachers, and impacts undergraduate students from diverse colleges in the region.

该合同为购买混合式四极杆飞行时间串联质谱仪(Q-TOF)提供资金，该质谱仪与完全集成的超高压液相色谱(UPLC)系统相连接。该仪器将是授予包括康奈尔蛋白质组学核心设施在内的六个主要用户和多个次要用户的研究资助NSF研究补助金的核心。该仪器将补充蛋白质组学核心设施和植物生物系的现有设备，特别是通过将其应用于蛋白质-蛋白质和蛋白质-配体相互作用的分析以及对基因组序列未知的生物的蛋白质进行从头测序。高灵敏度和高精度的串联质谱仪在低丰度蛋白质的鉴定和表征中是至关重要的。此外，该仪器将是使用稳定同位素标记进行比较定量(植物)蛋白质组学研究的关键工具。质谱仪将加速和促进康奈尔大学不同院系的广泛研究项目。该仪器预计还将帮助培训40多名本科生、研究生和博士后，并将在美国国家科学基金会资助的一个名为“植物基因组学”的推广计划中发挥作用，该计划吸引了高中生和教师，并影响了该地区不同大学的本科生。