Phosphoproteomic analysis of the rice XA21-Mediated Resistance Response

水稻 XA21 介导的抗性反应的磷酸化蛋白质组学分析

• 批准号: 0817738
• 负责人: Scott Peck
• 金额: $ 65.05万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0817738&HistoricalAwards=false
• 关键词: Phosphoproteomic; analysis; rice; XA21; Mediated

As sessile organisms, plants must constantly defend themselves from potential microbial pathogens. For protection, plants have developed effective defense mechanisms initiated by the extracellular detection of the potential pathogen. However, the signaling pathways that translate extracellular recognition of pathogens into the plant?s resistance are poorly understood. Therefore, identification and characterization of individual signaling components in a cereal crop such as rice will lead to a broader understanding of the defense response in all cereals. The project will use advanced biochemical approaches to identify signaling components activated in rice plants resistant to the bacterial pathogen that causes bacterial leaf blight, a devastating disease of rice throughout the world. Reverse genetic approaches will be used to test if these newly identified signaling components play a significant role in mediating resistance against the bacterial pathogen. The project is expected to identify at least 10-20 new signaling components and to test by reverse genetics 10 of the most promising candidates for their role in resistance against pathogens. Improving knowledge about how the plant responds to invasion by potential pathogens is critical for improving plant yields and quality. Moreover, rice is the leading genomics system for the agriculturally important cereals. Therefore, results from this study will directly benefit researchers studying pathogen stress in other cereal crops. In addition, the project will be used to train undergraduate students in state of the art biochemical techniques.

作为固着生物，植物必须不断地保护自己免受潜在的微生物病原体的侵害。为了保护，植物已经发展了有效的防御机制，通过细胞外检测潜在的病原体来启动。然而，将病原体的细胞外识别翻译到植物中的信号通路是什么？的阻力知之甚少。因此，在谷类作物，如水稻中的单个信号组分的鉴定和表征将导致在所有谷物中的防御反应的更广泛的理解。该项目将使用先进的生物化学方法来识别水稻植物中激活的信号成分，这些水稻植物对导致细菌性叶枯病的细菌性病原体具有抗性，细菌性叶枯病是一种世界各地的水稻毁灭性疾病。反向遗传方法将用于测试这些新鉴定的信号传导组分是否在介导对细菌病原体的抗性中起重要作用。该项目预计将确定至少10-20种新的信号成分，并通过反向遗传学测试10种最有希望的候选成分在抵抗病原体方面的作用。提高植物对潜在病原体入侵的反应知识对于提高植物产量和质量至关重要。此外，水稻是农业上重要谷物的主要基因组学系统。因此，这项研究的结果将直接有利于研究其他谷类作物病原体胁迫的研究人员。 此外，该项目还将用于培养本科生掌握最先进的生物化学技术。