High Throughput Nanoimprint Manufacturing of Shape-Specific, Stimuli-Responsive Polymeric Nanocarriers for Drug and Imaging Agent Delivery

用于药物和显像剂输送的形状特异性、刺激响应性聚合物纳米载体的高通量纳米压印制造

• 批准号: 0900715
• 负责人: Li Shi
• 金额: $ 90万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0900715&HistoricalAwards=false
• 关键词: Throughput; Nanoimprint; Manufacturing; Shape; Specific

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).The objective of this research is to develop nanomanufacturing methods for fabrication of shape-specific ?smart? nanoparticles capable of delivering drugs or imaging agents to targeted tissues in response to disease-specific or physiological signals. Specifically, high throughput, bio-compatible nanoimprint manufacturing processes are developed in this research to fabricate highly monodisperse, enzymatically-triggered nanoscale carriers of drug and imaging agents. The sizes and shapes of the nanoparticles are controlled during the top-down nano-imprint process. Experiments are carried out to evaluate nanoparticle loading, the release of drug and imaging agents, and to characterize the effects of nano-carrier size and shape on carrier transport and cellular uptake in cell cultures and microfluidic environments.This research can transform the manufacturing of nanoparticles for drug and imaging agent delivery as well as address fundamental questions regarding the optimal size and shape of nano-carriers. The obtained nano-carriers can significantly improve therapeutic care of complex diseases such as cancer or cardiovascular diseases. Moreover, the results from this research would not only provide new directions in fabricating drug delivery vehicles with disease-responsive properties, but would also explore the fundamental limitations and practical capabilities of generating three-dimensional, complex structures with nanomanufacturing techniques. If successful, this would eventually lead to the next generation of disease-specific and highly effective therapeutics and also provide novel biomedical applications for nanoimprint lithography. The project is inherently interdisciplinary and involves principles from manufacturing, mechanical, and biomedical engineering. This provides a unique and rewarding educational environment for the students involved including students from underrepresented groups in engineering professions. The results and concepts developed here directly benefit several graduate and undergraduate courses and are disseminated into industry and public by the active participations of the investigators in short courses and seminars for industry and K-12 teachers, students, and parents.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。这项研究的目的是开发纳米制造方法，用于制造形状特定的？聪明吗能够响应疾病特异性或生理信号将药物或成像剂递送至靶组织的纳米颗粒。具体而言，高通量，生物相容性纳米压印制造工艺在这项研究中开发，以制造高度单分散，酶促触发的药物和成像剂的纳米级载体。纳米颗粒的尺寸和形状在自顶向下的纳米压印过程中受到控制。实验进行评估纳米颗粒负载，药物和成像剂的释放，并表征纳米载体的大小和形状对载体运输和细胞摄取的影响，在细胞培养和微流体环境中，这项研究可以改变用于药物和成像剂递送的纳米颗粒的制造，以及解决有关纳米载体的最佳大小和形状的基本问题。所获得的纳米载体可以显著改善复杂疾病如癌症或心血管疾病的治疗护理。此外，这项研究的结果不仅将为制造具有疾病响应特性的药物递送载体提供新的方向，而且还将探索利用纳米制造技术生成三维复杂结构的基本限制和实用能力。如果成功，这将最终导致下一代疾病特异性和高效的治疗方法，并为纳米压印光刻提供新的生物医学应用。该项目本质上是跨学科的，涉及制造，机械和生物医学工程的原则。这为参与的学生提供了一个独特而有益的教育环境，包括来自工程专业代表性不足群体的学生。这里开发的结果和概念直接受益于几个研究生和本科生课程，并传播到行业和公众的短期课程和研讨会的调查人员的积极参与，为行业和K-12教师，学生和家长。