Effects of Veterinary Medicines on the Structural Diversity of the Microbial Community in the Rhizosphere under the Impact of temporal Moisture Gradients- GradMic

瞬时水分梯度影响下兽药对根际微生物群落结构多样性的影响- GradMic

• 批准号: 201560330
• 负责人: Professor Dr. Sören Thiele-Bruhn
• 金额: --
• 依托单位: Fach Bodenkunde
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/201560330?language=en
• 关键词: Effects; Veterinary; Medicines; Structural; Diversity

Medicinal antibiotics exert adverse effects on soil microbial biomass, activity, structural and functional diversity, and resistance level on a mid-term of weeks to months. By FOR566 this was confirmed for the field scale and at field conditions. However, environmental variables, especially soil moisture, alter the antibiotic effect to a yet unknown extent, hampering data interpretation. In the rhizosphere, representing a hot-spot of microbial abundance and activity, effects on structural diversity were determined when targeting specific microbial groups. Furthermore it was found that the specific investigation of root sections is required to unravel effects of antibiotics in this heterogeneous micro-habitat. Hence, it is proposed to investigate effects of sulfadiazine (SDZ) on the soil microbial community structure with special regard to (A) the impact of temporal moisture gradients and rewetting on the susceptibility of the microbial community and (B) effects of SDZ in rhizosphere soil and at the rhizoplane of apical vs. mature root sections. This will be done in a threefold approach by: (i) characterization of the temporal dynamics of antibiotic effects modulated by moisture gradients by intensive time resolved sampling, (ii) obtaining qualitative insights into effects on community structure through in-creased analysis on a group-specific level, and (iii) quantitative investigation of effects on community structure using real-time PCR.

药用抗生素对土壤微生物生物量、活性、结构和功能多样性以及抗性水平的中期影响可持续数周至数月。通过FOR566，在现场规模和现场条件下证实了这一点。然而，环境变量，特别是土壤湿度，改变抗生素的效果，但未知的程度，阻碍数据的解释。在根际，代表了一个热点的微生物丰度和活动，结构多样性的影响时，针对特定的微生物群体进行了测定。此外，人们发现，需要根部分的具体调查，以解开抗生素在这种异质微生境的影响。因此，建议研究磺胺嘧啶（SDZ）对土壤微生物群落结构的影响，特别是（A）时间水分梯度和再润湿对微生物群落敏感性的影响和（B）SDZ在根际土壤和根面的顶端与成熟根段的影响。这将通过三重方法完成：（i）通过密集的时间分辨采样来表征由水分梯度调制的抗生素效应的时间动态，（ii）通过在特定组水平上增加分析来获得对群落结构影响的定性见解，以及（iii）使用实时PCR定量研究对群落结构的影响。

项目成果

Soil microbial community responses to antibiotic-contaminated manure under different soil moisture regimes

• DOI: 10.1007/s00253-014-5717-4
• 发表时间: 2014-04
• 期刊: Applied Microbiology and Biotechnology
• 影响因子: 5
• 作者: Rüdiger Reichel;V. Radl;Ingrid Rosendahl;A. Albert;W. Amelung;M. Schloter;S. Thiele-Bruhn

Effects of slurry from sulfadiazine- (SDZ) and difloxacin- (DIF) medicated pigs on the structural diversity of microorganisms in bulk and rhizosphere soil

• DOI: 10.1016/j.soilbio.2013.03.007
• 发表时间: 2013-07-01
• 期刊: SOIL BIOLOGY & BIOCHEMISTRY
• 影响因子: 9.7
• 作者: Reichel, Ruediger;Rosendahl, Ingrid;Thiele-Bruhn, Soeren
• 通讯作者: Thiele-Bruhn, Soeren

Effects of sulfadiazine-contaminated fresh and stored manure on a soil microbial community

• DOI: 10.1016/j.ejsobi.2010.10.004
• 发表时间: 2011-01-01
• 期刊: EUROPEAN JOURNAL OF SOIL BIOLOGY
• 影响因子: 4.2
• 作者: Hammesfahr, Ute;Kotzerke, Anja;Thiele-Bruhn, Soeren
• 通讯作者: Thiele-Bruhn, Soeren

Effect of Sulfadiazine on Abundance and Diversity of Denitrifying Bacteria by Determining nirK and nirS Genes in Two Arable Soils

• DOI: 10.1007/s00248-010-9691-9
• 发表时间: 2010-06
• 期刊: Microbial Ecology
• 影响因子: 3.6
• 作者: K. Kleineidam;Shilpi Sharma;Anja Kotzerke;H. Heuer;S. Thiele-Bruhn;K. Smalla;B. Wilke;M. Schloter

Soil microbial community responses to sulfadiazine-contaminated manure in different soil microhabitats

• DOI: 10.1016/j.apsoil.2014.03.010
• 发表时间: 2014-08
• 期刊: Applied Soil Ecology
• 影响因子: 4.8
• 作者: Rüdiger Reichel;Diana Patzelt;Christoph Barleben;Ingrid Rosendahl;R. Ellerbrock;S. Thiele-Bruhn