CAREER: Paper-based surface enhanced Raman spectroscopy (P-SERS) for biosensing using inkjet-fabricated devices

该计划的目的是创建一个新的和变革类的侧向流生物分析微系统的基础上喷墨制造和纸基表面增强拉曼光谱（P-SERS）的多分析物检测。虽然纸基传感器最近出现了简单的分析，提出的方法，制造生物功能化的表面和使用喷墨打印的光流Sers检测元件，使灵敏和复杂的分析，是非常低的成本和简单的执行。这一努力的智力价值是建立的基本组成部分，使新的方法出现在复杂的纸基分析。这些组成部分包括：（i）基于喷墨的纤维素纸与生物分子和等离子体纳米结构的功能化，（ii）与金属纳米结构高度反应的拉曼报告分子，以及（iii）定量了解基于纸的流体生物传感器的质量传输优势。这项研究的更广泛的影响集中在P-SERS范式实现的生物分析的变革性方法上。P-SERS微系统的成本极低，加上前所未有的易用性，将导致广泛的新应用，这些应用需要高灵敏度，低成本的一次性分析设备，包括灾难响应，家庭医疗保健和传染病筛查。此外，P-SERS技术的独特性能使其能够在任何学习阶段和任何环境中作为教育工具。围绕喷墨制造和基于纸张的分析建立的研究机会和工程设计项目将为代表性不足的高中生和本科工程专业的学生创建。

The objective of this program is to create a new and transformative class of lateral-flow bioanalytical microsystems based on inkjet fabrication and paper-based surface enhanced Raman spectroscopy (P-SERS) for multi-analyte detection. While paper-based sensors have recently emerged for simplistic assays, the proposed approach of fabricating bio-functionalized surfaces and optofluidic SERS detection elements using inkjet printing enables sensitive and sophisticated assays that are extraordinarily low in cost and simple to perform.The intellectual merit of this effort is the establishment of the fundamental components that enable the emergence of new methodologies in sophisticated paper-based assays. These components include: (i) inkjet-based functionalization of cellulose paper with biomolecules andplasmonic nanostructures, (ii) Raman reporter molecules that are highly reactive with metal nanostructures, and (iii) a quantitative understanding of the mass transport advantages of paper-based fluidic biosensors.The broader impacts of this research are centered around the transformative approach to bioanalytics enabled by the P-SERS paradigm. The incredibly low cost of the proposed P-SERSmicrosystems combined with the unprecedented ease of use will lead to a broad range of new applications that require highly sensitive, low-cost disposable analytical devices, including applications in disaster response, at-home health care, and infectious disease screening. In addition, the unique properties of the P-SERS technology enable it to be incorporated as an educational tool at all stages of learning and in any environment. Research opportunities and engineering design projects built around inkjet fabrication and paper-based analytics will be created for underrepresented high school students and for undergraduate engineering students.