CAREER: Paper-based surface enhanced Raman spectroscopy (P-SERS) for biosensing using inkjet-fabricated devices

该计划的目标是创建一种基于喷墨制造和纸基表面增强拉曼光谱(P-SERS)的新型变革性横向流动生物分析微系统，用于多分析物检测。尽管纸基传感器最近出现了用于简单分析的方法，但使用喷墨打印制造生物功能化表面和光流控SERS检测元件的拟议方法使敏感和复杂的分析能够以极低的成本和简单的操作实现。这一努力的智力价值是建立了基本组件，使复杂的纸基分析出现了新的方法学。这些部分包括：(I)基于喷墨的纤维素纸与生物分子和等离子体纳米结构的功能化，(Ii)与金属纳米结构高度反应的拉曼报告分子，以及(Iii)对纸基流体生物传感器的质量传输优势的定量了解。这项研究的更广泛的影响集中在P-SERS范式实现的生物分析的变革性方法上。拟议的P-SERS微系统的成本低得令人难以置信，再加上前所未有的易用性，将导致广泛的新应用，这些应用需要高灵敏度、低成本的一次性分析设备，包括灾难应对、家庭保健和传染病筛查应用。此外，P-SERS技术的独特性质使其能够作为一种教育工具纳入学习的所有阶段和任何环境中。围绕喷墨制造和纸基分析的研究机会和工程设计项目将为未被充分代表的高中生和工科本科生创造。

The objective of this program is to create a new and transformative class of lateral-flow bioanalytical microsystems based on inkjet fabrication and paper-based surface enhanced Raman spectroscopy (P-SERS) for multi-analyte detection. While paper-based sensors have recently emerged for simplistic assays, the proposed approach of fabricating bio-functionalized surfaces and optofluidic SERS detection elements using inkjet printing enables sensitive and sophisticated assays that are extraordinarily low in cost and simple to perform.The intellectual merit of this effort is the establishment of the fundamental components that enable the emergence of new methodologies in sophisticated paper-based assays. These components include: (i) inkjet-based functionalization of cellulose paper with biomolecules andplasmonic nanostructures, (ii) Raman reporter molecules that are highly reactive with metal nanostructures, and (iii) a quantitative understanding of the mass transport advantages of paper-based fluidic biosensors.The broader impacts of this research are centered around the transformative approach to bioanalytics enabled by the P-SERS paradigm. The incredibly low cost of the proposed P-SERSmicrosystems combined with the unprecedented ease of use will lead to a broad range of new applications that require highly sensitive, low-cost disposable analytical devices, including applications in disaster response, at-home health care, and infectious disease screening. In addition, the unique properties of the P-SERS technology enable it to be incorporated as an educational tool at all stages of learning and in any environment. Research opportunities and engineering design projects built around inkjet fabrication and paper-based analytics will be created for underrepresented high school students and for undergraduate engineering students.