MRI: Acquisition of a Liquid Chromatography/Mass Spectrometer for Accurate Mass Determination and Complex Mixture Analysis

在主要研究仪器计划（MRI）和化学研究仪器计划（CRIF）的支持下，来自里本学院的Patrick Willoughby教授和同事Colleen Byron和Robert Wallace将获得一台能够提供精确质量的液相色谱仪，飞行时间质谱仪（lcms - tof精确质量）。一般来说，质谱（MS）是用于鉴定和表征嵌入在复杂基质中的少量化学物质的关键分析方法之一。在一个典型的实验中，这些成分流入质谱仪，在那里它们被电离成母离子和母离子的碎片离子，并测量它们的质量。这种高度灵敏的技术可以检测和确定复杂混合物中的分子结构。带有液相色谱仪的仪器通过在混合物到达质谱仪之前将其分离来提供额外的结构识别能力。作为一个主要的本科生机构，里彭的教师将本科生的研究经历作为他们研究活动最重要的成果之一。拟议的仪器将直接影响大量学生在三年项目期间的研究经验，并在未来几年更多。本科研究人员将与高中一起参加活动。这台仪器还将与威斯康星大学奥什科什分校以及劳伦斯大学的研究人员共享。该奖项旨在加强各级的研究和教育，特别是在以下领域：(a)开发涉及酰基自由基和光氧化还原催化的新的有机合成方法；(b)邻苯与羰基基的1,3-偶极环加成反应的优化条件；(c)研究活性氧副产物对电子转移蛋白的影响；(d)研究脂质分布对轮虫存活和轮虫菌落难食性的影响。

With this award from the Major Research Instrumentation Program (MRI) and support from the Chemistry Research Instrumentation Program (CRIF), Professor Patrick Willoughby from Ripon College and colleagues Colleen Byron and Robert Wallace will acquire a liquid chromatograph, time-of-flight mass spectrometer capable of providing accurate mass (LCMS-TOF-accurate mass). In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with a liquid chromatograph provides additional structural identification power by separating mixtures of compounds before they reach the mass spectrometer. As a primarily undergraduate institution, Ripon's faculty embraces undergraduate research experiences as one of the most important outcomes of their research activities. The proposed instrument will directly impact the research experiences of a large number of students within the three-year project period, and many more in years to come. Undergraduate researchers will participate in activities with high schools. This instrument will also be shared with researchers from the University of Wisconsin Oshkosh as well as those from Lawrence University.The award is aimed at enhancing research and education at all levels, especially in areas such as (a) developing new organic synthesis methodology involving acyl radicals and photoredox catalysis; (b) optimizing conditions for the 1,3-dipolar cycloaddition reaction of o-benzyne with a carbonyl ylide; (c) study the effects of byproducts of reactive oxygen species on electron-transferring proteins; and (d) studying the effect of lipid profiles on rotifer survival and the unpalatability of rotifer colonies.