MRI: Development of an Advanced Bio-Imaging Instrument: Enabling 3D quantitative multifunctional sensing at the nanoscale
MRI: Development of an Advanced Bio-Imaging Instrument: Enabling 3D quantitative multifunctional sensing at the nanoscale
批准号:
1429782
负责人:
Rafael Piestun
金额:
$66.88万
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
2014
资助国家:
美国
项目状态:
已结题
起止时间:
2014-08-15 至 2018-07-31
中文摘要
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英文摘要
An award is made to the University of Colorado at Boulder to develop next-generation optical nano-imaging instrumentation to expand research and research training in criticalareas of science and engineering. The project will transform the microscopy capabilities at the University of Colorado at Boulder by generating a multi-functional nanoscope with unique characteristics. The instrument will be shared by investigators in the BioFrontiers Institute and departments in the colleges of Engineering and Arts and Sciences. Wide availability of next generation optical nanoscopy is critical to ensure emerging interdisciplinary research activities. This platform will bring together physicists, electrical engineers, biological engineers, biochemists, molecular biologists, cellular biologists, computer scientists, and mathematicians to work together on developing methods for increasing resolution and advancing modern microscopy in biologically relevant problems. The equipment will be the center of interdisciplinary research and a focus for the development of new ideas and technologies that cross traditional departmental boundaries. Furthermore, the platform will provide state-of-the-art optical imaging capabilities to the community through the BioFrontiers core facility.The project will advance understanding of nanoscale phenomena both at the subcellular level and within different types of materials, bringing about improvements in bioscience and advanced instrumentation. Current bioscience research is strongly dependent onthe investigators ability to characterize sub-cellular structure and function at the nanoscale. Emerging optical microscopy techniques enable imaging of cellular details and macromolecular structure previously unimaginable. Remarkably, fluorescencenanoscopy is compatible with live cells and able to multiplex labeling with high molecular specificity. While these techniques promise to create a revolution in biological research, current incarnations present significant shortcomings: limited field of view and depth of field, limited acquisition rate unsuitable for fast live cell phenomena, need for significant light dosage creating photo-damage, and unsuitability for deep imaging. The project is set to overcome these shortcomings by an integrated design of techniques such as point-spread function engineering, single-molecule localization microscopy, fast structured illumination, and adaptive microscopy.
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IDBR Type B: Point-spread function engineered parallel scanning optical subsystem for fast quantitative high-resolution and high-sensitivity 3D imaging
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批准号:1556473
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项目类别:Continuing Grant
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资助金额:$44.9万
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财政年份:2016
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负责人:Rafael Piestun
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依托单位:
Non-invasive, high-resolution, 3D imaging and sensing through highly scattering materials
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批准号:1611513
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项目类别:Standard Grant
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资助金额:$41.0万
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财政年份:2016
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负责人:Rafael Piestun
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依托单位:
A new paradigm in optical design: infinitely linear refraction artificial materials
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批准号:1310487
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项目类别:Standard Grant
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资助金额:$36.0万
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财政年份:2013
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负责人:Rafael Piestun
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依托单位:
IDBR: Development and Dissemination of a Flexible Multifunctional Widefield 3D Superresolution Microscopy System for Quantitative Biological Research
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批准号:1063407
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项目类别:Continuing Grant
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资助金额:$35.67万
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财政年份:2011
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负责人:Rafael Piestun
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依托单位:
Aperiodic Volume Optics
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批准号:1028714
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项目类别:Standard Grant
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资助金额:$36.0万
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财政年份:2010
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负责人:Rafael Piestun
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依托单位:
Collaborative Research: Multimode Adaptive 3D Microscopy for Quantitative Analysis of Live-Cellular Dynamic Processes in Thick Samples
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批准号:0852885
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项目类别:Standard Grant
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资助金额:$50.0万
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财政年份:2009
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负责人:Rafael Piestun
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依托单位:
IGERT: Interdisciplinary Graduate Education in Computational Optical Sensing and Imaging
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批准号:0801680
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项目类别:Continuing Grant
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资助金额:$320.0万
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财政年份:2008
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负责人:Rafael Piestun
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依托单位:
NIRT: Three-Dimensional Nanomanufacturing Processes for Nanophotonic Devices and Systems
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批准号:0304650
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项目类别:Standard Grant
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资助金额:$100.0万
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财政年份:2003
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负责人:Rafael Piestun
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依托单位:
Integrated Sensing: High-speed 3D Microscopy by Hybrid Optical-Digital Encoding and Processing
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批准号:0225533
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项目类别:Continuing Grant
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资助金额:$25.0万
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财政年份:2002
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负责人:Rafael Piestun
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依托单位:
国内基金
海外基金
水稻边界发育缺陷突变体abnormal boundary development(abd)的基因克隆与功能分析
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批准号:32070202
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项目类别:面上项目
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资助金额:58.0万元
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批准年份:2020
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负责人:汪泉
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依托单位:
Development of a Linear Stochastic Model for Wind Field Reconstruction from Limited Measurement Data
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批准号:--
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项目类别:--
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资助金额:40万元
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批准年份:2020
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负责人:Vikrant Gupta
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依托单位: