Dissertation Research: Intra-population genomic and metabolic diversity among understudied archaea in methane-cycling wetlands

论文研究：甲烷循环湿地中待研究古细菌的种群内基因组和代谢多样性

• 批准号: 1701970
• 负责人: Christopher Miller
• 金额: $ 2.02万
• 依托单位: University of Colorado at Denver-Downtown Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1701970&HistoricalAwards=false
• 关键词: Dissertation; Research; Intra; population; genomic

Archaea are microorganisms that are known to play critical roles in carbon and nitrogen cycling, but little is known about the diversity and evolution of the group. Woesearchaeota, is a poorly known group of Archaea that is commonly found in freshwater wetlands but can also be found in diverse ecosystems globally. As with most members of Archaea, there are no cultured representatives to study in the laboratory, and few available genomes sequences to help infer function. This research will employ environmental DNA sequencing methods and associated computational approaches to generate multiple novel Woesearchaeota genomes from a freshwater wetland. Analysis of these genomes will advance knowledge of the functional potential of this group globally. This work will provide new insights into the role of Archaea in regulating biogeochemical processes. One graduate student will be trained in molecular systematics and undergraduates will receive hands-on research experience on the fundamentals of scientific computing and bioinformatics. This research will produce novel whole-genome sequences and inferences about the metabolic potential for the recently described archaeal group, Woesearchaeota. To-date only a handful of Woesearchaeota genomes and partial genomes have been reconstructed. A high-resolution census of Archaea in a freshwater wetland has revealed that Woesearchaeota at this site are phylogenetically diverse, and comprise two ecologically differentiated ecotypes with niches defined by site geochemistry. The hypothesis that observed niche preferences can be explained by genomic variation will be tested by targeted reconstruction of multiple Woesearchaeota genomes. Researchers will also test the hypotheses that local evolutionary diversifications have resulted in a small core genome, and that the high phylogenetic diversity observed within this phylum is mirrored by high levels of inferred functional diversity.

古生菌是已知在碳和氮循环中发挥关键作用的微生物，但对该类群的多样性和进化知之甚少。Woesearchaeota，是一种鲜为人知的古生物群，常见于淡水湿地，但也可以在全球不同的生态系统中找到。就像古生代的大多数成员一样，没有培养的代表可以在实验室进行研究，也没有可用的基因组序列来帮助推断功能。这项研究将使用环境DNA测序方法和相关的计算方法，从淡水湿地产生多个新的Woesearchaeota基因组。对这些基因组的分析将在全球范围内推进对这一群体功能潜力的了解。这项工作将为古生代在调节生物地球化学过程中的作用提供新的见解。一名研究生将接受分子系统学培训，本科生将获得科学计算和生物信息学基础的实践研究经验。这项研究将产生新的全基因组序列，并推断最近描述的古菌群Woesearchaeota的代谢潜力。到目前为止，只重建了为数不多的灰毛虫基因组和部分基因组。对淡水湿地中的古生物进行了高分辨率的普查，结果表明，该地点的灰指肠动物在系统发育上是多样化的，包括两种不同的生态型，其生态位由地点地球化学定义。观察到的生态位偏好可以通过基因组变异来解释的假设将通过对多个Woesearchaeota基因组的有针对性的重建来检验。研究人员还将检验这样的假设，即局部进化多样化导致了一个较小的核心基因组，并且在这个门中观察到的高系统发育多样性反映了高水平的推断功能多样性。