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SHF: Small: Development and Manufacturing Integrated DNA Circuits

SHF：小型：开发和制造集成 DNA 电路

基本信息

批准号：
1907824
负责人：
Dmitry Kolpashchikov
金额：
$ 29.67万
依托单位：
The University of Central Florida Board of Trustees
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2019
资助国家：
美国
起止时间：
2019-10-01 至 2023-09-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1907824&HistoricalAwards=false
关键词：
SHF Small Development Manufacturing Integrated

项目摘要

Electronic computers have dramatically increased the efficiency of information processing and enabled automation of industrial processes. Such computers, however, have limitations when applied to address biomedical tasks including medical diagnostics and therapy. To recognize and process biological inputs, such as DNA, proteins, and small biomolecules, electronic computers require chemical-sensor intermediates that convert a chemical input into an electronic output. Computers are hard to insert into a human body as they are made of non-biocompatible and non-biodegradable materials. Biomedical applications of computational technologies would get another dimension if computers are made from a biocompatible material. Such computers could operate in a point-of-care diagnostic test by recognizing and autonomously analyzing complex combinations of biochemical disease markers, which would facilitate medical diagnostics, making them more accurate and affordable. In addition, such computers would help building molecular robots -- nanometer-scale devices that can sense biological markers inside human body to diagnose a disorder and promptly address it, which would help doctors fight such devastating diseases as cancer and viral infections, among others.DNA has attracted growing attention as a material for constructing molecular computational devices. Its parallel data-processing capabilities and predictability of Watson-Crick base pairing poses a fascinating opportunity for building computer and molecular robots from DNA. Moreover, biocompatibility of such devices, as well as previously developed approaches for gene therapy, would enable their applications in diagnostics and treatment of cancer, infectious and genetic diseases. The long-term goal of this project is to construct a molecular-scale processor based on DNA logic gates and apply it to address biomedical challenges. In this project, PI proposes to address the following technological challenges in a quest to the develop a first DNA computer: (i) create multilayered integrated circuits (DNA nanochips); and (ii) stabilize DNA nanochips by covalent cross-link. In addition, the PI will develop DNA nanochips to solve a practically significant biomedical problem: recognition of cancer markers, which is expected to stimulate interest in DNA computation. The proposed developments will be showcased by creating a user-friendly construction kit for building integrated DNA circuits, which can be used in educational and outreach projects. The practical utility of the kits will be tested in laboratory settings in collaboration with high-school students from a local Orlando high school.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

电子计算机极大地提高了信息处理的效率，实现了工业过程的自动化。然而，这种计算机在应用于包括医疗诊断和治疗在内的生物医学任务时具有局限性。为了识别和处理生物输入，如DNA、蛋白质和生物小分子，电子计算机需要化学传感器中间体，将化学输入转换为电子输出。计算机很难植入人体，因为它们是由非生物兼容和不可生物降解的材料制成的。如果计算机是由生物兼容材料制成的，那么计算技术的生物医学应用将获得另一个维度。这类计算机可以通过识别和自主分析生化疾病标志物的复杂组合，在医疗保健点诊断测试中运行，这将促进医疗诊断，使其更准确、更负担得起。此外，这类计算机将有助于制造分子机器人--一种纳米级的设备，可以感应人体内的生物标记来诊断疾病并迅速解决问题，这将帮助医生抗击癌症和病毒感染等毁灭性疾病。DNA作为构建分子计算设备的材料吸引了越来越多的关注。它的并行数据处理能力和沃森-克里克碱基配对的可预测性为用DNA建造计算机和分子机器人提供了一个迷人的机会。此外，这些设备的生物兼容性，以及以前开发的基因治疗方法，将使它们能够应用于癌症、传染病和遗传病的诊断和治疗。该项目的长期目标是构建一种基于DNA逻辑门的分子规模处理器，并将其应用于应对生物医学挑战。在这个项目中，PI建议解决以下技术挑战，以探索开发第一台DNA计算机：(I)创造多层集成电路(DNA纳米芯片)；以及(Ii)通过共价交联稳定DNA纳米芯片。此外，PI将开发DNA纳米芯片，以解决一个实际意义重大的生物医学问题：癌症标记物的识别，这有望激发人们对DNA计算的兴趣。拟议的发展将通过创建一种用户友好的建造集成电路DNA电路的工具包来展示，该工具包可用于教育和外联项目。这些试剂盒的实用性将在实验室环境中与奥兰多当地一所高中的学生合作进行测试。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。