Sensor-integrated 3D tissue scaffold for physiological interrogations

用于生理询问的传感器集成 3D 组织支架

• 批准号: 1917630
• 负责人: Zheng Yan
• 金额: $ 36.63万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1917630&HistoricalAwards=false
• 关键词: Sensor; integrated; 3D; tissue; scaffold

Cells and tissues are often cultured outside the living environment (in vitro) to facilitate various studies in disease modeling, drug test and precision medicine. The mechanical and electrical behaviors of a tissue system are two basic physiological properties that indicate the tissue state. Consequently, the real-time monitoring of both parameters in the 3D tissue is important for assessing tissue state and revealing biological mechanisms. A comprehensive assessment requires the knowledge of both parameters at distributed locations buried deep in the tissue, which remains challenging. This project aims to integrate multifunctional bioelectronic sensors in a 3D macroporous scaffold, in which the scaffold provides a 3D microenvironment for tissue culturing whereas the distributed and embedded sensors can real-time monitor both electrical and mechanical responses from the tissue. The developed system can lead to more precise biomedical devices for disease modeling, drug screening, and health diagnostics. The outreach efforts are expected to broaden STEM participation and education. The interdisciplinary research and training will prepare next-generation young minds to entrepreneurial experiences through "Innovation-Challenge Competition". Plans are to create "Women Engineer Day" which will host 15-20 high school girls and to add "Eureka!" program to target middle to high school girls. Understanding physiological and pathological behaviors of live cells in deep tissues can provide both fundamental insights into biological mechanisms and biomedical solutions to diseases. Conventional biosensing technologies, such as optical imaging and planar biochips, are often confined to surface regions. To transcend these limitations, this project aims to develop and validate a novel type of 3D, sensor-innervated, electronic scaffold systems which can enable high-speed and simultaneous measurements of both bioelectrical and biomechanical signals within engineered tissues. The central approach is to employ multi-level hierarchical assemblies to integrate multifunctional biosensors in a programmable 3D scaffold. The developed system will provide a new tool to fundamental studies in cell mechanics and electrophysiology. The research is also expected to lead to translational biochips that can upgrade current single-parameter, planar physiological quantification into multi-parameter, deep-tissue physiological quantifications in engineered tissues.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

细胞和组织通常在生活环境外（体外）培养，以促进疾病建模，药物测试和精准医学的各种研究。组织系统的力学和电学行为是表征组织状态的两个基本生理特性。因此，实时监测三维组织中的两个参数对于评估组织状态和揭示生物机制是重要的。全面的评估需要了解深埋在组织中的分布式位置处的两个参数，这仍然具有挑战性。该项目旨在将多功能生物电子传感器集成到3D大孔支架中，其中支架为组织培养提供3D微环境，而分布式和嵌入式传感器可以实时监测组织的电学和机械响应。开发的系统可以为疾病建模，药物筛选和健康诊断带来更精确的生物医学设备。预计外联工作将扩大STEM参与和教育。跨学科的研究和培训将通过“创新挑战赛”为下一代年轻人提供创业经验。 计划设立“女工程师日”，将有15-20名高中女生参加，并增加“尤里卡！“针对初中到高中女生的计划。了解深部组织中活细胞的生理和病理行为可以提供生物学机制的基本见解和疾病的生物医学解决方案。传统的生物传感技术，如光学成像和平面生物芯片，往往局限于表面区域。为了超越这些限制，该项目旨在开发和验证一种新型的3D，传感器神经支配的电子支架系统，该系统可以实现工程组织内生物电信号和生物力学信号的高速和同时测量。核心方法是采用多级分层组件将多功能生物传感器集成在可编程的3D支架中。该系统为细胞力学和电生理学的基础研究提供了新的工具。该研究还有望导致转化生物芯片，可以升级目前的单参数，平面生理量化到多参数，深组织生理量化在工程组织。这一奖项反映了NSF的法定使命，并已被认为是值得通过评估使用基金会的智力价值和更广泛的影响审查标准的支持。

项目成果

Multifunctional Protein Nanowire Humidity Sensors for Green Wearable Electronics

• DOI: 10.1002/aelm.202000721
• 发表时间: 2020-08-12
• 期刊: ADVANCED ELECTRONIC MATERIALS
• 影响因子: 6.2
• 作者: Liu, Xiaomeng;Fu, Tianda;Yao, Jun
• 通讯作者: Yao, Jun

Bioelectronic protein nanowire sensors for ammonia detection

• DOI: 10.1007/s12274-020-2825-6
• 发表时间: 2020-05-11
• 期刊: NANO RESEARCH
• 影响因子: 9.9
• 作者: Smith, Alexander F.;Liu, Xiaomeng;Yao, Jun
• 通讯作者: Yao, Jun

Laser-scribed conductive, photoactive transition metal oxide on soft elastomers for Janus on-skin electronics and soft actuators

用于 Janus 皮肤电子器件和软执行器的软弹性体上的激光划线导电、光活性过渡金属氧化物

• DOI: 10.1126/sciadv.abp973
• 发表时间: 2022
• 期刊: Science advances
• 影响因子: 13.6
• 作者: Zhao, G.;Ling, Y.;Su, Y.;Chen, Zanyu;Mathai, C.;Emeje, O.;Brown, A.;Alla, D.;Huang, J.;Kim, C.
• 通讯作者: Kim, C.

Laser-scribed conductive, photoactive transition metal oxide on soft elastomers for Janus on-skin electronics and soft actuators.

• DOI: 10.1126/sciadv.abp9734
• 发表时间: 2022-06-24
• 期刊: Science advances
• 影响因子: 13.6