800 MHz NMR-Spektrometerkonsole mit Kryoplattform

带低温平台的 800 MHz NMR 波谱仪控制台

• 批准号: 379418483
• 金额: --
• 依托单位: Universität Regensburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/379418483?language=en
• 关键词: 800; MHz; NMR; Spektrometerkonsole; mit

The primary scientific goal of our research is to understand how large molecular machines function. We mainly focus on enzymes and large protein-RNA complexes that play a role in mRNA degradation. This essential biological process is part of gene-expression as the life-time of a transcript correlates with cellular protein levels. In our studies, we go well beyond determining static structural information and aim to obtain detailed insights into transient intermolecular interactions, protein dynamics and functionally relevant conformational changes. This is especially important for enzymes that have to undergo structural rearrangements to perform biological tasks. Experimentally we make extensive use of high-resolution NMR-spectroscopy that is ideally suited to detect and quantify dynamic processes and with atomic resolution in a near cellular setting. The assemblies that we study often have a molecular weight far over 100 kDa, which is an order of magnitude larger than systems that are routinely studied. We therefore exploit and develop novel methods in NMR spectroscopy and combine these with specialized isotope labeling schemes. Due to the challenging nature of our studies, we rely on highly sensitive and state-of-the-art NMR hardware. An upgrade of the NMR hardware at the Regensburg University is thus essential for the future success of our research.

我们研究的主要科学目标是了解大分子机器是如何工作的。我们主要关注在mRNA降解中起作用的酶和大的蛋白质-RNA复合体。这一重要的生物学过程是基因表达的一部分，因为转录本的寿命与细胞蛋白质水平相关。在我们的研究中，我们远远超出了确定静态结构信息的范围，目标是获得对瞬时分子间相互作用、蛋白质动力学和功能相关构象变化的详细见解。这对于必须经历结构重排才能执行生物任务的酶来说尤其重要。在实验上，我们广泛使用高分辨率核磁共振光谱学，它非常适合于检测和量化动态过程，并在接近细胞的环境中具有原子分辨率。我们研究的组件通常具有远远超过100 kDa的分子量，这比常规研究的系统大一个数量级。因此，我们开发和开发了核磁共振光谱学的新方法，并将这些方法与专门的同位素标记方案相结合。由于我们的研究具有挑战性，我们依赖于高灵敏度和最先进的核磁共振硬件。因此，升级雷根斯堡大学的核磁共振硬件对我们未来的研究成功至关重要。