800 MHz NMR-Spektrometerkonsole mit Kryoplattform

我们研究的主要科学目标是了解大型分子机器的功能。我们主要关注在mRNA降解中发挥作用的酶和大蛋白质-RNA复合物。这个基本的生物过程是基因表达的一部分，因为转录本的寿命与细胞蛋白质水平相关。在我们的研究中，我们远远超出了确定静态结构信息，旨在获得对瞬时分子间相互作用，蛋白质动力学和功能相关构象变化的详细见解。这对于必须经历结构重排以执行生物任务的酶尤其重要。实验上，我们广泛使用高分辨率NMR光谱，非常适合检测和量化动态过程，并在近细胞环境中具有原子分辨率。我们研究的组件通常具有远超过100 kDa的分子量，这比常规研究的系统大一个数量级。因此，我们开发和开发新的方法，在核磁共振波谱和联合收割机这些与专门的同位素标记计划。由于我们的研究具有挑战性，我们依赖于高灵敏度和最先进的NMR硬件。因此，在里根斯堡大学的NMR硬件的升级是必不可少的，我们的研究的未来成功。

The primary scientific goal of our research is to understand how large molecular machines function. We mainly focus on enzymes and large protein-RNA complexes that play a role in mRNA degradation. This essential biological process is part of gene-expression as the life-time of a transcript correlates with cellular protein levels. In our studies, we go well beyond determining static structural information and aim to obtain detailed insights into transient intermolecular interactions, protein dynamics and functionally relevant conformational changes. This is especially important for enzymes that have to undergo structural rearrangements to perform biological tasks. Experimentally we make extensive use of high-resolution NMR-spectroscopy that is ideally suited to detect and quantify dynamic processes and with atomic resolution in a near cellular setting. The assemblies that we study often have a molecular weight far over 100 kDa, which is an order of magnitude larger than systems that are routinely studied. We therefore exploit and develop novel methods in NMR spectroscopy and combine these with specialized isotope labeling schemes. Due to the challenging nature of our studies, we rely on highly sensitive and state-of-the-art NMR hardware. An upgrade of the NMR hardware at the Regensburg University is thus essential for the future success of our research.