Development of a highly efficient, lower cellular damage gene transfer method by a spheroid formation of blood cells for regenerative medicine and cellular therapy

通过血细胞球体形成开发高效、低细胞损伤的基因转移方法，用于再生医学和细胞治疗

• 批准号: 23659609
• 负责人: KATANO Hisako
• 金额: $ 2.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23659609/
• 关键词: トランスレーショナルリサーチ; ウイルスベクター; 遺伝子治療; 細胞療法; 樹状細胞

Using a model of gene transduction into dendritic cells with recombinant adenoviral vector expressing EGFP, I developed an improved gene transfer method with spheroid formation, which avoided low efficient and/or high cellular damage through conventional methods. It could be a now method of a highly efficient, lower cellular damage gene transfer by a spheroid formation for blood cells for regenerative medicine and cellular therapy.

利用腺病毒介导的EGFP基因转染树突状细胞的模型，建立了一种改进的球状体基因转染方法，避免了传统方法的低效和/或高细胞损伤。它可能是一种高效、低细胞损伤的基因转移方法，通过球状体形成血细胞，用于再生医学和细胞治疗。