Construction of Detection System for Ligand Screening in Solution Using Peptide Generation based on Molecular Interactions

为了开发一种在溶液中筛选能够与蛋白质结合的配体的系统，我们试图在配体-蛋白质相互作用发生时，在带有配体的肽与蛋白质之间构建连接反应。此外，利用所生成的肽，可以产生酶活性。通过模型相互作用对、螺旋状肽和核糖核酸酶S重构，我们成功地证明了该检测系统可以很好地工作。

In order to develop a screening system in solution for ligands capable of binding to proteins, we have attempted to construct a ligation reaction between peptides bearing a ligand and a protein when the ligand-protein interaction occurs. Moreover, using the generated peptide, enzymatic activity can be generated. Using a model interaction pair, coiled-coil peptides, and ribonuclease S reconstitution, we have successfully demonstrated that the detection system can work well.