Exploitation of a tool for analyzing molecular interactions on the plasma membrane by using membrane-bound antibodies

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We cloned cDNAs for anti-HA and anti-Myc monoclonal antibodies, and made membrane-bound antibody constructs, which were verified to function as antibodies against HA- and Myc-tagged proteins. We further made single-stranded antibodies, which were verified to function as antibodies. Using membrane-bound antibodies, we next tried to measure the tension between two adjacent cells in Xenopus embryos by using the FRET-based tension sensor module (TSMod). In addition, we found that development of Xenopus gastrulae is affected when cells are bridged with the membrane-bound antibodies and membrane-bound antigens. This bridging method can be used as a tool for investigation of intercellular plasticity during morphogenesis.