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Design and Synthesis of DNA-Cleaving Molecules

Design and Synthesis of DNA-Cleaving Molecules
DNA 切割分子的设计与合成
批准号:
02453023
负责人:
SAITO Isao
金额:
$3.33万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (B)
财政年份:
1990
资助国家:
日本
项目状态:
已结题
起止时间:
1990 至 1991

项目摘要

项目成果

SAITO Isao的其他基金

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中文摘要
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英文摘要
There has been great interest in designing reagents that bind to DNA duplex selectively at desired sequences. Such molecules equipped with a chemical or photochemical tag capable of modifying or cleaving DNA at the site of binding should have great prospects as "synthetic artificial restriction enzymes" and not least as gene-selective drugs. We have focused on the use of natural DNA-binders such as antitumor antibiotics, DNA-binding proteins or sequence-defined oligonucleotides in combination with photoactivated DNA-cleaving molecules. We have succeeded in designing a new sequence specific "photochemical DNA cleaver" that binds specifically to-5'-GG-3'sequence. This molecule named as "photo-Fenton-reagent" is widely used as a convenient source of hydroxyl radical. We also succeeded in designing a different type of DNA-cleaving molecules based on the conceit obtained from the work on the action mechanism of ene-diyne type antitumor antibiotics such as neocarzinostatin or esperamicin.
期刊论文(52)
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会议论文
杉山 弘: "Specific Detection of Cー4'Hydroxylated Abasic Sites Generated by Bleomycin and Neocarzinostatin in DNA" J.Am.Chem.Soc.112. 5252-5257 (1990)
Hiroshi Sugiyama:“DNA 中博来霉素和新制癌菌素生成的 C-4 羟基化无碱基位点的特异性检测”J.Am.Chem.Soc.112 (1990)。
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通讯作者:
杉本 弘: "Specific Detection of Cー4' Hydroxylated Abasic Sites Generated by Bleomycin and Neocarzinostatin in DNA" J.Am.Chem.Soc.112. 5250-5257 (1990)
Hiroshi Sugimoto:“DNA 中博来霉素和新制癌菌素生成的 C-4 羟基化无碱基位点的特异性检测”J.Am.Chem.Soc.112 (1990)。
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永田 龍: "DNA Cleavage by Acyclic EneyneーAllene Systems related to Neocarzinostatin and EsperamicinーCalicheamicin" Tetrahedron Lett.31. 2907-2910 (1990)
Ryu Nagata:“与新制癌菌素和埃斯波霉素-卡利车霉素相关的无环烯-联烯系统的 DNA 切割”Tetrahedron Lett.31 (1990)。
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杉山 弘: "Highly Sequence Selective Photreaction of 5ーBromouracilーContaining deoxyhexanucleotide" J.Am.Chem.Soc.112. 6720-6721 (1990)
Hiroshi Sugiyama:“含有 5-溴尿嘧啶的脱氧六核苷酸的高度序列选择性光反应”J.Am.Chem.Soc.112 6720-6721 (1990)。
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21
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