Gene Manipulation using Laser
使用激光进行基因操作
基本信息
- 批准号:13308033
- 负责人:
- 金额:$ 27.12万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (A)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We synthesized various vinyluracil analogs from 5-iododeoxyuridine-in a high yield. Vinyluracils were converted to the phosphoramidite form, and then incorporated to 5' end of oligodeoxynucleotides (ODNs). Using the vinyluracil-containing ODN, photoligation was carried out in the presence of the ODN containing pyrimidine base at 3' end and the template ODN. The photoligation efficiency was quantified using polyacrylamide gel electrophoresis. The pyrimidine possessing a carboxyvinyl group exhibited 10 times higher photoligation reactivity than 5-vinyluridine. We also found an efficient synthetic route for vinyluracil analogs. After the ODN containing 5-formyluracil was prepared, ODNs possessing vinyluracil analogs were synthesized using Wittig reaction for 5-formyluracil.
我们以5-碘脱氧尿苷为原料合成了多种乙烯基尿嘧啶类似物。将乙烯基尿嘧啶转化为亚磷酰胺形式,然后掺入到寡脱氧核苷酸(ODN)的5'末端。使用含乙烯基尿嘧啶的ODN,在3'端含嘧啶碱基的ODN和模板ODN的存在下进行光连接。使用聚丙烯酰胺凝胶电泳定量光连接效率。具有羧基乙烯基的嘧啶表现出比5-乙烯基尿苷高10倍的光连接反应性。我们还发现了一个有效的合成路线乙烯基尿嘧啶类似物。在制备含5-甲酰尿嘧啶的ODN后,利用Wittig反应合成含乙烯基尿嘧啶类似物的ODN。
项目成果
期刊论文数量(17)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Chikara Dohno, Atsusi Ogawa, Kazuhiko Nakatand, Isao Saito: "Hole Trapping at N^6-Cyclopropyldeoxyadenosine Suggests a Direct Contribution Of Adenine Bases to Hole Transport through DNA"JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 125(34). 10154-10155 (2003)
Chikara Dohno、Atsusi Okawa、Kazuhiko Nakatand、Isao Saito:“N^6-环丙基脱氧腺苷上的空穴捕获表明腺嘌呤碱基对通过 DNA 进行的空穴传输有直接贡献”《美国化学会杂志》。
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Chikara Dohno, Atsusi Ogawa, Kazuhiko Nakata, Isao Saito: "Hole Trapping at N^6-Cyclopropyldeoxyadenosine Suggests a Direct Contribution Of Adenine Bases to Hole Transport through DNA."JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 125(34). 10154-10156 (2003)
Chikara Dohno、Atsusi Okawa、Kazuhiko Nakata、Isao Saito:“N^6-环丙基脱氧腺苷的空穴捕获表明腺嘌呤碱基对通过 DNA 的空穴运输有直接贡献。”美国化学会杂志。
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- 影响因子:0
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Akimitu Okamot, Kazuki Tainaka, Isao Saito: "Clear Distinction of Purine Bases on the Complementary Strand by a Fluorescence Change of a Novel Fluorescent Nucleoside."JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 125(17). 4972-4973 (2003)
Akimitu Okamot、Kazuki Tainaka、Isao Saito:“通过新型荧光核苷的荧光变化清楚地区分互补链上的嘌呤碱基。”美国化学会杂志。
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- 影响因子:0
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Kazuhiko Nakatani, Souta Horie, Isao Saito: "Affinity Labeling of Single Guanine Bulge"J.Am.Chem.Soc.. 125(30). 8972-8973 (2003)
Kazuhiko Nakatani、Souta Horie、Isao Saito:“单个鸟嘌呤突出物的亲和标记”J.Am.Chem.Soc.. 125(30)。
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- 影响因子:0
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- 通讯作者:
Chikara Dohno, Atsishi Ogawa, Kazuhiko Nakatani, Isao Saito: "Hole Trapping at N6-Cyclopropyldeoxyadenosine Suggests a Direct Contribution of Adenine Bases to Hole Transport through DNA"J.Am.Chem.Soc.. 125(34). 10154-10155 (2003)
Chikara Dohno、Atsishi Okawa、Kazuhiko Nakatani、Isao Saito:“N6-环丙基脱氧腺苷上的空穴捕获表明腺嘌呤碱基对 DNA 空穴传输的直接贡献”J.Am.Chem.Soc. 125(34)。
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- 影响因子:0
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SAITO Isao其他文献
Simultaneous determination of urinary pyrethroid insecticide metabolites in disposable diaper samples
一次性尿布样品中尿拟除虫菊酯类杀虫剂代谢物的同时测定
- DOI:
- 发表时间:
2012 - 期刊:
- 影响因子:0
- 作者:
UEYAMA Jun;SAITO Shun;KONDO Takaaki;SAITO Isao;GOTOH Masahiro;NAKAI Kunihiko;KAMIJIMA Michihiro - 通讯作者:
KAMIJIMA Michihiro
Quantitation of neonicotinoid metabolites in human urine using gas chromatography-mass spectrometry
使用气相色谱-质谱法定量人尿液中的新烟碱类代谢物
- DOI:
- 发表时间:
2013 - 期刊:
- 影响因子:0
- 作者:
NOMURA Hiroshi;UEYAMA Jun;KONDO Takaaki;SAITO Isao;MURATA Katsuyuki;IWATA Toyoto;WAKUSAWA Shinya;KAMIJIMA Michihiro - 通讯作者:
KAMIJIMA Michihiro
Concentration distribution of urinary pyrethroid insecticide metabolites in Japanese workers
日本工人尿液中拟除虫菊酯类杀虫剂代谢物浓度分布
- DOI:
- 发表时间:
2013 - 期刊:
- 影响因子:0
- 作者:
UEYAMA Jun;NOMURA Hiroshi;SAITO Isao;KONDO Takaaki;SUGIURA Yuka;MURATA Katsuyuki;IWATA Toyoto;GOTOH Masahiro;WAKUSAWA Shinya;KAMIJIMA Michihiro - 通讯作者:
KAMIJIMA Michihiro
SAITO Isao的其他文献
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{{ truncateString('SAITO Isao', 18)}}的其他基金
Design of Environmentally Sensitive Fluorescent Nucleosides and Their Application to Innovative Fluorescence Probe for Gene Analysis
环境敏感荧光核苷的设计及其在基因分析创新荧光探针中的应用
- 批准号:
23350082 - 财政年份:2011
- 资助金额:
$ 27.12万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Evaluation for primary prevention of cardiovascular disease in a community.
社区心血管疾病一级预防评价。
- 批准号:
23590795 - 财政年份:2011
- 资助金额:
$ 27.12万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Evaluation of the pattern of the skeletal deviation by the simulation of bone remodeling.
通过模拟骨重塑来评估骨骼偏差的模式。
- 批准号:
22592281 - 财政年份:2010
- 资助金额:
$ 27.12万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Epidemiological study on the association between the autonomic function and glucose intolerance
植物神经功能与糖耐量异常关系的流行病学研究
- 批准号:
20590647 - 财政年份:2008
- 资助金额:
$ 27.12万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Evaluation of the process of the mandibular deviation by the simulation of bone remodeling.
通过模拟骨重建评价下颌偏斜的过程。
- 批准号:
19592348 - 财政年份:2007
- 资助金额:
$ 27.12万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Design of Base-discriminating Fluorescent Nucleosides and Their Application to Gene Analysis and SNP Genotyping
碱基识别荧光核苷的设计及其在基因分析和 SNP 基因分型中的应用
- 批准号:
18201026 - 财政年份:2006
- 资助金额:
$ 27.12万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Possible effects of various neuropeptides on cells related to bone resorption during tooth movement
牙齿移动过程中各种神经肽对骨吸收相关细胞的可能影响
- 批准号:
12671992 - 财政年份:2000
- 资助金额:
$ 27.12万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Geo-ecological Study on the "Transfer" and Diffusion of Cultivated Crops in Japan
日本栽培作物“转移”与扩散的地生态学研究
- 批准号:
11480015 - 财政年份:1999
- 资助金额:
$ 27.12万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Alterations in expression of neuropeptide gene in trigeminal ganglion in orthodontic tooth movement
正畸牙移动过程中三叉神经节神经肽基因表达的变化
- 批准号:
09672099 - 财政年份:1997
- 资助金额:
$ 27.12万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular Design of Autitumor Autibiotic by DNA double Crosslinking.
通过 DNA 双交联进行自肿瘤自生素的分子设计。
- 批准号:
08555228 - 财政年份:1996
- 资助金额:
$ 27.12万 - 项目类别:
Grant-in-Aid for Scientific Research (A)