Possible effects of various neuropeptides on cells related to bone resorption during tooth movement

牙齿移动过程中各种神经肽对骨吸收相关细胞的可能影响

• 批准号: 12671992
• 负责人: SAITO Isao
• 金额: $ 2.11万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671992/
• 关键词: Tooth movement; Cells related to bone resorption; Osteoblasts; CGRP; Treatment efficiency; HSP; カルシトニン遺伝子関連ペプチド; VIP

In this study, we investigated altered binding of neouropeptides such as CGRP to PDL cells including osteoclasts during experimental tooth movement. Heat Shock Protein (HSP)-immunoreactivity in the rat PDL and its change were also examined immunohistochemically since the orthodontic force had been thought to possibly induce alterations in the expression of stress protein like HSP. 50-day-old Wistar strain rats were used. Experimental tooth movement was performed according to Waldo's method.Each animal was euthanized at each appointed time. After decalcification, serial horizontal frozen sections, 40 μ in thickness were cut. Free-floating sections were processed for the ABC method by the use of an antiserum to each substance such as CGRP, Substance P or HSP.The following results were obtained :1. The nerve fibers containing CGRP and/or Substance P observed under physiological condition decreased or disappeared immediately after the onset of treatment, and then began to increase gradually a few days later again.2. CGRP-immunoreactivity was seen in the alveolar osteoblasts on the surface of newly formed osteoid in the tension site. Both CGRP- and Substance P-immunoreactivity were observed in some osteoclasts existing in the Hawship lacunae in the compression site. On the other hand, In vitro, addition of CGRP to cultured osteoclasts caused inhibition of osteoclastic activity.3. Immunohistochemical staining intensity for HSP in the PDL showed significant change during tooth movement, suggesting that HSP may be associated with defensive responses against physical or chemical stimuli with orthodontic tooth movement.

在这项研究中，我们研究了改变结合的新尿肽，如降钙素基因相关肽PDL细胞，包括破骨细胞在实验性牙齿移动。热休克蛋白（HSP）的免疫反应性在大鼠牙周膜及其变化，因为正畸力已被认为可能会引起变化的应激蛋白，如HSP的表达也进行了化学检测。使用50日龄Wistar系大鼠。实验性牙齿移动按Waldo方法进行，每只动物在指定时间处死。脱钙后取40 μ厚连续冰冻切片。用抗CGRP、P物质、HSP的抗血清处理自由漂浮切片，作ABC法。生理状态下观察到的含CGRP和/或P物质的神经纤维在治疗开始后立即减少或消失，几天后又开始逐渐增加. CGRP免疫反应阳性细胞位于张力区新生类骨质表面的牙槽骨成骨细胞。CGRP和P物质的免疫反应，观察到一些破骨细胞存在于Hawship陷窝的压缩网站。另一方面，在体外培养的破骨细胞中加入CGRP可抑制破骨细胞的破骨活性. HSP在牙周膜中的免疫组化染色强度在牙齿移动过程中有明显变化，提示HSP可能与正畸牙齿移动时对物理或化学刺激的防御反应有关。

项目成果

Ishii, Kazuhiro: "Effects of Hotz' plate-based two-stage palatoplasty in unilateral cleft lip and palate -A comparative study of subjects treated with one-stage palatoplasty and normal subjects-"Orthodontic Waves (J. Jpn. Orthod. Soc). 59(6). 402-406 (200

Ishii, Kazuhiro：“Hotz基于板的两阶段腭成形术对单侧唇腭裂的影响 - 一阶段腭成形术治疗受试者与正常受试者的比较研究 -”Orthodontic Waves (J. Jpn. Orthod. Soc)

Isao SAITO: "Considerations in efficient orthodontic treatment : Changes in bone formation and/or osteoblastic activity in response to mechanical stress such as orthodontic force"J. Kohokushinetsu Orthod. Soc.. 9 (1). 19-24 (2001)

Isao SAITO：“有效正畸治疗的考虑因素：响应机械应力（例如正畸力）的骨形成和/或成骨细胞活性的变化”J.

Kuniyasu HIROSHIMA: "Temporal and spatial distribution of Fos protein in the parabranchial nucleus neurons during experimental tooth movement of the rat molars"Brain Research. 908. 161-173 (2001)

Kuniyasu HIROSHIMA：“大鼠磨牙实验性牙齿运动过程中鳃旁核神经元中 Fos 蛋白的时空分布”脑研究。

Kazuhiro ISHII: "Effects of Hotz' plate-based two -stage palatoplasty in unilateral cleft Lip and palate-A comparative study of sutjects treated with one-stage palatoplasty and normal subjects-"Orthodontic Waves. 59 (6). 402-406 (2000)

Kazuhiro ISHII：“Hotz基于板的两阶段腭成形术对单侧唇腭裂的影响-一阶段腭成形术治疗受试者与正常受试者的比较研究-”正畸波。

斉藤 功: "矯正治療の効率化を考える-特に矯正力に対する骨形成ならびに骨芽細胞の動態に着目して-"甲北信越矯正歯科学会雑誌. 9(1). 19-24 (2001)

Isao Saito：“思考提高正畸治疗的效率 - 特别关注骨形成和成骨细胞响应正畸力的动态 -”Kohoku Shinetsu 正畸学会杂志 9(1)。