Identification of a Chromosome Carrying a Putative Tumor Suppressor gene in Human Choriocarcinoma by Microcell-Mediated Chromosome Transfer.
通过微细胞介导的染色体转移鉴定人绒毛膜癌中携带假定肿瘤抑制基因的染色体。
基本信息
- 批准号:63480363
- 负责人:
- 金额:$ 3.2万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1988
- 资助国家:日本
- 起止时间:1988 至 1990
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
There are two main mechanisms of origin for complete hydatidiform mole ; a) fertilization of an empty egg by a haploid sperm followed by duplication and b) fertilization of such an egg by two haploid spermatozoa. It is widely accepted that of all forms of pregnancy that had to choriocarcinoma the risk associated with moles is by far the highest. Homozygous expression of a recessive mutation of moles has been assumed to associate with this propensity to malignancies.Alterations of several tumor suppressor genes together with activation of oncogenes are assumed to be necessary for choriocarcinogenesis. Genetic characteristics shown in the moles suggest that the former alterations are especially important to explore the multistep conversions to malignancies. Thus, individual chromosomes derived from normal cells were introduced into choriocarcinoma cells via microcell fusion. Evaluation of tumorigenicity in microcell hybrids suggested that chromosome #7 carried a putative tumor suppressor gene for choriocarcinoma. The gene imprinting or recessive mutation mmy be responsible for the inactivation of such a gene. Being able to correspond with the high propensity to malignancy of moles. Now, the cDNA expression liberaties specific to the normal #7 chromosome could be produced. Colony hybridization using mRNA probes obtained from normal villi and CCI choriarcinoma in addition to suicide selection cloning the revertant will be able to clone the tumor suppressor genes of choriocarcinoma.
完全性葡萄胎有两种主要的起源机制:a)用单倍体精子受精空卵,然后进行复制;b)用两个单倍体精子受精。人们普遍认为,到目前为止,在所有不得不患绒毛膜癌的妊娠中,与葡萄胎相关的风险最高。葡萄胎隐性突变的纯合子表达被认为与这种恶性倾向有关。几个肿瘤抑制基因的改变和癌基因的激活被认为是绒毛膜癌发生所必需的。葡萄胎的遗传特征表明,前者的改变对于探索多步转化为恶性肿瘤特别重要。因此,来自正常细胞的单个染色体通过微细胞融合被导入绒毛膜癌细胞。微细胞杂交瘤的致瘤性评估表明,7号染色体携带一个可能的绒毛膜癌抑癌基因。基因印记或隐性突变可能是导致这种基因失活的原因。能够与鼹鼠的高度恶变倾向相对应。现在,可以生产出正常7号染色体特异的cDNA表达自由。利用从正常绒毛和CCI绒毛膜癌组织中获得的mRNA探针进行菌落杂交,除自杀选择克隆外,该突变株将能够克隆绒毛膜癌抑癌基因。
项目成果
期刊论文数量(63)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
藤野敬史,和気徳夫,加藤秀則,藤本征一郎: "4.ヒト癌遺伝子研究の動向とDNA診断 12)絨毛癌 胞状奇胎" 日本臨床. 589. 695-700 (1989)
Takashi Fujino、Norio Wake、Hidenori Kato、Seiichiro Fujimoto:“4.人类癌症基因研究和 DNA 诊断的趋势 12) 绒毛膜癌葡萄胎”日本临床。 589. 695-700 (1989)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Wake,N.,Yamada,H.,Fujita,H.et al: "lsolation of clone resistant to 6ーthioguanine and G418 from the HHUA endometrial carcinoma cells and their application to cell hybridization." Cancer Genetics and Cytogenetics. 49. 117-123 (1990)
Wake, N.、Yamada, H.、Fujita, H. 等人:“从 HHUA 子宫内膜癌细胞中分离出对 6-硫鸟嘌呤和 G418 的克隆抗性及其在细胞杂交中的应用。”49。117。 -123 (1990)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
山田 秀人: "細胞融合法を用いた子宮内膜癌化機構解明へのアプロ-チ" 日本産科婦人科学会雑誌. 41. 122-128 (1989)
Hideto Yamada:“使用细胞融合方法阐明子宫内膜癌发生机制的方法”日本妇产科学会杂志 41. 122-128 (1989)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
和気 徳夫: "胞状奇胎からの絨毛癌化ーその機構と研究の現状ー" オンコロジア. 24. 31-39 (1991)
Norio Wake:“葡萄胎引起的绒毛膜癌 - 其机制和研究现状”《肿瘤学》24. 31-39 (1991)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
WAKE Norio其他文献
Transcriptional factors, DEC1 and DEC2 cooperatively regulate epithelial-to-mesenchymal transition of uterine endometrial cancer cells.
转录因子DEC1和DEC2协同调节子宫内膜癌细胞的上皮间质转化。
- DOI:
- 发表时间:
2014 - 期刊:
- 影响因子:0
- 作者:
ASANOMA Kazuo;KOBAYASHI Hiroaki;WAKE Norio;KATO Kiyoko - 通讯作者:
KATO Kiyoko
WAKE Norio的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('WAKE Norio', 18)}}的其他基金
Genome diversity associated with in montalization and establishment of endometrial cancer stem cell isolation
与子宫内膜癌干细胞分离的蒙塔化和建立相关的基因组多样性
- 批准号:
20390435 - 财政年份:2008
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular targeted therapy by inducing cancer cell senesence
通过诱导癌细胞衰老的分子靶向治疗
- 批准号:
14104014 - 财政年份:2002
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
Molecular mechanism of endometrial carcinoma development and their application for the new molecular target therapy
子宫内膜癌发生的分子机制及其在新型分子靶向治疗中的应用
- 批准号:
12470344 - 财政年份:2000
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanism of cell senescence
细胞衰老的分子机制
- 批准号:
11557121 - 财政年份:1999
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanism of endometrial cancer and its application to gene diagnosis
子宫内膜癌的分子机制及其在基因诊断中的应用
- 批准号:
09470362 - 财政年份:1997
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of new therapy for uterine cervical carcinoma
宫颈癌新疗法的开发
- 批准号:
08557092 - 财政年份:1996
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Establishment of gene diagnosis and therapy for Endometrial carcinoma.
子宫内膜癌基因诊断和治疗的建立。
- 批准号:
07457391 - 财政年份:1995
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Genetic Events Associated With Choriocarcinogenesis.
与绒毛膜癌发生相关的遗传事件。
- 批准号:
07042006 - 财政年份:1995
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for international Scientific Research
Development of gene diagnosis and therapy for endometrial cancers.
子宫内膜癌基因诊断和治疗的发展。
- 批准号:
05454453 - 财政年份:1993
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Application of antisense oligo DNA to uterine cancers.
反义寡DNA在子宫癌中的应用。
- 批准号:
05557073 - 财政年份:1993
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
相似海外基金
Establishment of a comprehensive cancer immunotherapy based on whole-genome analysis for treatment-resistant choriocarcinoma
建立基于全基因组分析的针对难治性绒毛膜癌的综合癌症免疫疗法
- 批准号:
20K09639 - 财政年份:2020
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Investigation about LAMP-2 which could be a noble therapeutic target against choriocarcinoma
LAMP-2可能成为绒毛膜癌高贵治疗靶点的研究
- 批准号:
18K16764 - 财政年份:2018
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
analysis of immune factor in choriocarcinoma microenvironment
绒毛膜癌微环境免疫因子分析
- 批准号:
17K16845 - 财政年份:2017
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
In vitro approaches to evaluate placental drug transport by using differentiating JEG-3 human choriocarcinoma cells
使用分化 JEG-3 人绒毛膜癌细胞评估胎盘药物转运的体外方法
- 批准号:
23590207 - 财政年份:2011
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The mechanism of carcinogenesis of choriocarcinoma with glycosyltransferases for hyperglycosylated hCG
高糖基化 hCG 糖基转移酶致癌绒毛膜癌的机制
- 批准号:
23592445 - 财政年份:2011
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The functional analysis of the putative choriocarcinoma suppressor gene, HTF12 in choriocarcinoma.
假定的绒毛膜癌抑制基因 HTF12 在绒毛膜癌中的功能分析。
- 批准号:
15591759 - 财政年份:2003
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The molecular mechanism and morphological change of oncogenesis of choriocarcinoma
绒毛膜癌发生的分子机制及形态学变化
- 批准号:
13671726 - 财政年份:2001
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The analysis of OS-4 like gene and homeo protein like gene as a putative choriocarcinoma suppressor gene.
OS-4样基因和同源蛋白样基因作为假定的绒毛膜癌抑制基因的分析。
- 批准号:
13671728 - 财政年份:2001
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Isolation and Identification of Tumor suppressor gene in choriocarcinoma.
绒毛膜癌抑癌基因的分离与鉴定。
- 批准号:
11671631 - 财政年份:1999
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The analysis of genomic imprinting in ovarian teratomas and choriocarcinoma
卵巢畸胎瘤和绒毛膜癌的基因组印记分析
- 批准号:
08671888 - 财政年份:1996
- 资助金额:
$ 3.2万 - 项目类别:
Grant-in-Aid for Scientific Research (C)