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Epidemiological Studies on Biliary Tract Cancer in Niigata, Japan

日本新泻县胆道癌的流行病学研究

基本信息

批准号：
04670307
负责人：
YAMAMOTO Masaharu
金额：
$ 1.34万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Epidemiological studies of biliary tract cancer(BTC) have been conducted over the last decade, the rationale being that the deaths rates in Niigata Prefecture where we live were found to be the highest in Japan. The present study was conducted as a part of the epidemiological studies on BTC.The aim of the present study is to test a multifactorial causation hypothesis by making use of an animal model. In details, we designed a protocol on the basis of multifactorial causation hypothesis where a suspected chemical, chlornitrfen(CNP) is given to golden hamsters whose gallbladders are surgically operated to have "experimental gallstone".Five experimental groups were established as follows ; Group 1 : gallstone + normal diet, Group 2 : gallstone + CNP(5000ppm), Group 3 : positive carcinogen, 3-methylcholanthrene (3-MC) + nomal diet, Group 4 : 3-MC + CNP(5000ppm), Group 5(Control Group) : sham-oparation + nomal diet. Twenty female golden hamsters (9-10 weeks age at the time of initiation were used in each group. The periods of experiments were 72 weeks in the groups of 1,2 and 5, and 17 weeks in the groups of 3 and 4. CNP treatment in the group 2 was stopped at the 17th weeks and normal diet was introduced in the rest of the experimental period, since the body weight increase was affected as compared with the control group.During the course of experiment for 72 weeks in the groups of 1,2 and 5, three animals each were dead. In the groups of 3 and 4, none of them died for 17 weeks. At the time of sacrifice(72 weeks in groups of 1,2 and 5, and 17 weeks in the groups of 3 and 4), 1 and 2 cases of hyperplastic nodules in the liver were found in the groups of 1 and 2, respectively. In regards to the occurence of gallbladder cancer, the histopathological examination was being conducted and the details of the findings will be appeared elsewhere.

过去十年进行了胆道癌（BTC）的流行病学研究，其理由是我们居住的新泻县的死亡率被发现是日本最高的。本研究是 BTC 流行病学研究的一部分。本研究的目的是利用动物模型检验多因素因果假设。具体来说，我们根据多因素因果假设设计了一个方案，将一种可疑的化学物质氯硝苯（CNP）给予胆囊手术后患有“实验性胆结石”的金仓鼠。建立了五个实验组，如下；第1组：胆结石+正常饮食，第2组：胆结石+CNP(5000ppm)，第3组：阳性致癌物，3-甲基胆蒽(3-MC)+正常饮食，第4组：3-MC+CNP(5000ppm)，第5组(对照组)：假手术+正常饮食。每组使用20只雌性金黄仓鼠（开始时年龄为9-10周）。实验周期在1，2和5组中为72周，在3和4组中为17周。第2组中的CNP治疗在第17周时停止，并且在其余实验期间引入正常饮食，因为与对照组相比，体重增加受到影响。在实验过程中，72周小组中的周数 1,2和5中，各有三只动物死亡。在 3 组和 4 组中，17 周内没有人死亡。处死时（1，2组和5组为72周，3组和4组为17周），1组和2组分别发现1例和2例肝脏增生结节。关于胆囊癌的发生，正在进行组织病理学检查，详细情况调查结果将在其他地方发表。