Epidemiological Studies on Mutagenicity of Human Bile

用艾姆斯试验研究了人胆汁经蓝人造丝处理后的致突变性。在目前的研究中。两年来承担了两个主要的研究项目。一是分析日本胆囊癌死亡率最高和最低的新泻和高知县胆汁的致突变性。二是比较日本胆汁与智利铁木库胆汁的致突变活性，智利胆囊癌死亡率居世界首位。在第一次调查中，在新泻和高知采集了52和59份胆汁样本。采用鼠伤寒沙门菌TA98加S9mix的Ames法检测胆汁液蓝粘胶提取物的致突变性。52份高危人群标本中有32份（61.5%）具有诱变性。在我们之前的研究中，24份样本中有14份（58.3%）存在诱变性。将这些数据与本研究的结果结合起来，76个样本中有46个（60.5%）显示出突变性。另一方面，从低危人群中收集的59份样本中，仅检测到7份（11.9%）具有诱变性。因此，我们发现胆汁致突变性存在显著的地理差异。在第二项调查中，仅采集了女性胆石症患者的胆汁样本。智利Temuco市24份胆汁标本中，20份（83.3%）呈致突变性。日本胆汁26例中有21例（80.8%）具有诱变性，新泻和高知县26例中有5例（19.2%）具有诱变性。虽然新泻样品的诱变活性与智利的Temuco没有差异，但代表诱变能力的反向菌落数在新泻比Trmuco低；62 (SY。+ -。[] 14 vs 128 []]) 92(意思是美国南达科他州(SY。+ -)))。无论在平均数量上的差异，诱变活性都随着年龄的增长而增加。

The mutagenicity of human bile was investigated in the Ames test after blue rayon treatment. In the present study. Two main research projects have been undertaken for two years. One is to analyze the mutagenicity of bile collected from Niigata and Kochi, where the mortalities from the gallbladder cancer are the highest and the lowest in Japan, respectively. The other is compara the mutagenic activities of Japanese bile with those from Temuco, Chile, where the mortality from the gallbladder cancer has been the highest in the world.In the first investigation, 52 and 59 bile samples were collected from Niigata and Kochi. Mutagenicity of blue rayon extracts of bile juice was detected by Ames assay with Salmonella typhimurium TA98 with S9mix.Thirty-two (61.5%) of 52 samples obtained from the high risk population were mutagenic. In our previous study, the mutagenicity was observed in 14 (58.3%) of 24 samples. After combining this data with the results of the present study, 46 (60.5%) of 76 samples revealed the mutagenicity. On the other hand, the mutagenicity was detected in only 7 (11.9%) of 59 samples collected from the low risk population. Therefore, we found a significantgeographical difference in the bile mutagenicity.In the second investigation, bile samples were collected only from the female patients with cholelithiasis. Of 24 bile samples in Temuco, Chile, 20 (83.3%) showed the mutagenicity. In the case of Japanese bile, 21 (80.8%) of 26 and 5 (19.2%) of 26 were mutagenic in Niigata and Kochi, respectively. Although the mutagenic activity of Niigata samples does not differ from that of Temuco, Chile, the number of revertant colonies, which stands for the mutagenic power, is lower in Niigata than Trmuco ; 62(]SY.+-.])14 vs 128 (]SY.+-.])92 (mean(]SY.+-.])S.D.). Regardless of the differences in the average numbers of revertant colonies, mutagenic activity increased with age all in the three groups.