Epidemiological Studies on Mutagenicity of Human Bile

人胆汁致突变性的流行病学研究

• 批准号: 06670376
• 负责人: YAMAMOTO Masaharu
• 金额: $ 1.34万
• 依托单位: Niigata University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670376/
• 关键词: biliary tract cancer; gallbladder cancer; bile juice; mutagenicity; Ames法

The mutagenicity of human bile was investigated in the Ames test after blue rayon treatment. In the present study. Two main research projects have been undertaken for two years. One is to analyze the mutagenicity of bile collected from Niigata and Kochi, where the mortalities from the gallbladder cancer are the highest and the lowest in Japan, respectively. The other is compara the mutagenic activities of Japanese bile with those from Temuco, Chile, where the mortality from the gallbladder cancer has been the highest in the world.In the first investigation, 52 and 59 bile samples were collected from Niigata and Kochi. Mutagenicity of blue rayon extracts of bile juice was detected by Ames assay with Salmonella typhimurium TA98 with S9mix.Thirty-two (61.5%) of 52 samples obtained from the high risk population were mutagenic. In our previous study, the mutagenicity was observed in 14 (58.3%) of 24 samples. After combining this data with the results of the present study, 46 (60.5%) of 76 samples revealed the mutagenicity. On the other hand, the mutagenicity was detected in only 7 (11.9%) of 59 samples collected from the low risk population. Therefore, we found a significantgeographical difference in the bile mutagenicity.In the second investigation, bile samples were collected only from the female patients with cholelithiasis. Of 24 bile samples in Temuco, Chile, 20 (83.3%) showed the mutagenicity. In the case of Japanese bile, 21 (80.8%) of 26 and 5 (19.2%) of 26 were mutagenic in Niigata and Kochi, respectively. Although the mutagenic activity of Niigata samples does not differ from that of Temuco, Chile, the number of revertant colonies, which stands for the mutagenic power, is lower in Niigata than Trmuco ; 62(]SY.+-.])14 vs 128 (]SY.+-.])92 (mean(]SY.+-.])S.D.). Regardless of the differences in the average numbers of revertant colonies, mutagenic activity increased with age all in the three groups.

用艾姆斯试验研究了人胆汁经蓝色人造丝处理后的致突变性。在本研究中。两个主要的研究项目已经进行了两年。一是分析从新泻和高知收集的胆汁的致突变性，这两个地方的胆囊癌死亡率分别是日本最高和最低的。第一次调查分别从日本新泻和高知采集了52份和59份胆汁样品，并与胆囊癌死亡率居世界首位的智利Temuco的胆汁进行了比较。用鼠伤寒沙门氏菌TA 98加S9混合物作艾姆斯试验，对52份胆汁蓝丝提取物进行致突变性检测，检出32份（61.5%）。在我们以前的研究中，在24份样品中的14份（58.3%）中观察到致突变性。将这些数据与本研究的结果相结合后，76份样品中有46份（60.5%）显示出致突变性。另一方面，从低风险人群中采集的59份样品中仅7份（11.9%）检出致突变性。因此，我们发现胆汁致突变性存在明显的地理差异。智利特木科的24份胆汁样本中，20份（83.3%）显示出致突变性。对于日本胆汁，新泻和高知分别有21/26（80.8%）和5/26（19.2%）例具有致突变性。虽然新泻样品的致突变活性与智利Temuco样品的致突变活性没有差异，但代表致突变能力的回复突变菌落数在新泻中低于Trmuco; 62（] SY.+-.]）14对128（] SY.+-.]）92（平均值（] SY.+-.]）S.D.）。无论回复突变菌落的平均数量差异如何，三组中的致突变活性均随年龄增加。

项目成果

遠藤和男: "胆道がんの疫学" 新潟医学会雑誌. 108. 7-13 (1994)

远藤和夫：“胆道癌的流行病学”新泻医学会杂志 108. 7-13 (1994)。

Mano.H.et al.: "Geographical Variations in Mutagenicity of Blue Rayon Extracts of Japanese Human Bile" Mutation Research-Genetic Toxicology Testing.

Mano.H.等人：“日本人胆汁蓝色人造丝提取物致突变性的地理差异”突变研究-基因毒理学测试。

山本正治他: "胆嚢癌の疫学" 臨床消化器内科. 9. 1543-1551 (1994)

Masaharu Yamamoto 等人：“胆囊癌的流行病学”临床胃肠病学 9. 1543-1551 (1994)

中平浩人、山本正治他: "The Dsitribution of Selenium and Molybdenum and Cancer Mortality in Niigata,Japan" Archives of Environmental Health. 50. 374-380 (1995)

Hiroto Nakahira、Masaharu Yamamoto 等人：“日本新泻县硒和钼的分布与癌症死亡率”环境健康档案 50. 374-380 (1995)。

MANO,HIROSHI et al.: "Comaprisons of Mutagenic Activity of Bile between Japanese and Chilean Female Patients with Cholelithiasis by the Ames Test" Mutation Research. (投稿中). (1996)

MANO, HIROSHI 等人：“通过艾姆斯试验比较日本和智利女性胆石症患者胆汁的致突变活性”（正在进行）。