Epidemiological Studies on Free Fatty Acid Concentrations in Human Gallbladder Bile from the Point of View of Antimutagencity

建立了胆汁中游离脂肪酸(FFAs)的高效液相色谱分析方法。在该方法中，胆汁中的游离脂肪酸用异溶物柱一步提取，然后用9-芳基重氮酮(ADAM)衍生化。ADAM之所以被选中，是因为它在室温下与羧酸的反应活性很高。然后，用高效液相色谱法对游离脂肪酸进行分离和定量。实践证明，该方法简单、省时。人胆汁中FFA的平均回收率为97.6%，检出限为100-250pg。用该方法测定了11例胆结石患者的胆汁中游离脂肪酸的浓度。总游离脂肪酸平均浓度为0.61(SD=0.41)mm o l/L，个体间差异较大，与岛田等人的结果有一定差异。(1991年)。在他们的报告中，来自日本福冈胆结石患者的胆汁中FFA的总浓度为1.3(0.47)mmol/L(n=10)，是我们在新泻(0.61)mmol/L中获得的值的两倍。从地理肿瘤学的角度来看，FFA浓度的差异是特别有趣的，因为从地理肿瘤学的角度来看，FFA浓度的差异是特别令人感兴趣的，因为从地理肿瘤学的角度来看，福冈是胆囊癌的低风险地区，新泻是高危地区。游离脂肪酸的浓度或组成与胆囊癌发生之间的可能关系应通过世界各地的进一步研究来评估。

We developed a high-permance liquid chromatograohy (HPLC) method for free fatty acids (FFAs) analysis in bile. In this method, FFAs were extracted from bile in a single step using an Isolute ^<TM>ODS cartride, derivatized with 9-anthrydiazome than (ADAM). ADAM was chosen because of its high reactivity with carboxylic acid at room temperature. Then, HPLC was used for separating and quantifying FFAs. This method proved to be simple and time-saving. The mean recovery of FFA added to human gallbladder bile was 97.6% and the detection limit was 100-250 pg. Using this method, we determined FFA concentrations in the gallbladder bile of 11 gallstone patients. The mean concentration of total FFA was 0.61(SD=0.41) mmol/L,and there was wide variation in the individual FFAs.Our result are somewhat discrepant from those of Shimada et.al. (1991). In their reprt, the total FFAs concentration in gallbladder bile obtained from gallstone patients in Fukuoka, Japan, was 1.30 (0.47) mmol/L (n=10), that is, twice the values we obtained in Niigata (0.61) mmol/L.The discrepancy in FFA concentrations, dispite other conditions such as disease, age and sex being almost identical between the two reports, is of particular interest from the viewpoint of geographical oncology ; Fukuoka is know as a low-risk for gallbladder cancer, and Niigata as a high-risk area. The possible relationship between the concentration or composition of FFAs and occurrence of gallbladder cancer should be assessed by further studies in various parts of the wold.