Epidemiological Studies on Free Fatty Acid Concentrations in Human Gallbladder Bile from the Point of View of Antimutagencity

从抗诱变角度对人胆囊胆汁中游离脂肪酸浓度的流行病学研究

• 批准号: 08670387
• 负责人: YAMAMOTO Masaharu
• 金额: $ 1.6万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670387/
• 关键词: gallbladder cancer; bile; fatty acid; HPLC; mutagenicity; antimutagencity; 胆のうがん; 高速液体クロマトグラフィー; 突然変異原性

We developed a high-permance liquid chromatograohy (HPLC) method for free fatty acids (FFAs) analysis in bile. In this method, FFAs were extracted from bile in a single step using an Isolute ^<TM>ODS cartride, derivatized with 9-anthrydiazome than (ADAM). ADAM was chosen because of its high reactivity with carboxylic acid at room temperature. Then, HPLC was used for separating and quantifying FFAs. This method proved to be simple and time-saving. The mean recovery of FFA added to human gallbladder bile was 97.6% and the detection limit was 100-250 pg. Using this method, we determined FFA concentrations in the gallbladder bile of 11 gallstone patients. The mean concentration of total FFA was 0.61(SD=0.41) mmol/L,and there was wide variation in the individual FFAs.Our result are somewhat discrepant from those of Shimada et.al. (1991). In their reprt, the total FFAs concentration in gallbladder bile obtained from gallstone patients in Fukuoka, Japan, was 1.30 (0.47) mmol/L (n=10), that is, twice the values we obtained in Niigata (0.61) mmol/L.The discrepancy in FFA concentrations, dispite other conditions such as disease, age and sex being almost identical between the two reports, is of particular interest from the viewpoint of geographical oncology ; Fukuoka is know as a low-risk for gallbladder cancer, and Niigata as a high-risk area. The possible relationship between the concentration or composition of FFAs and occurrence of gallbladder cancer should be assessed by further studies in various parts of the wold.

建立了胆汁中游离脂肪酸的高效液相色谱分析方法。在该方法中，使用<TM>9-蒽二氮杂蒽（ADAM）衍生的Isolute ^ ODS萃取剂，在一个步骤中从胆汁中提取FFA。选择ADAM是因为其在室温下与羧酸的高反应性。然后，使用HPLC分离和定量FFA。这种方法被证明是简单和节省时间。游离脂肪酸的平均回收率为97.6%，检测限为100-250 pg。用此方法测定了11例胆囊结石患者胆囊胆汁中游离脂肪酸的浓度。总FFA浓度平均值为0.61（SD=0.41）mmol/L，个体间差异较大，与Shimada等（1991）的结果有一定差异。在他们的报告中，从日本福冈的胆结石患者中获得的胆囊胆汁中总FFA浓度为1.30（0.47）mmol/L（n=10），即我们在新泻获得的值的两倍（0.61）mmol/L，FFA浓度的差异，尽管其他条件如疾病、年龄和性别在两份报告中几乎相同，从地理肿瘤学的角度来看，福冈是胆囊癌的低风险地区，而新泻是高风险地区。游离脂肪酸的浓度或组成与胆囊癌发生之间的可能关系应通过在世界各地的进一步研究来评估。

项目成果

Kodama K, Nakadaira H, Endoh K, et al.: "Geographic clustering patterns in mortality from biliary tract cancer" Japanese Journal of Cancer Research. 89. 6-11 (1998)

Kodama K、Nakadaira H、Endoh K 等人：“胆道癌死亡率的地理聚类模式”日本癌症研究杂志。

Yamamoto M.: "Herbicides and gallbladder cancer in Japan" Reviews in Toxicology. 1. 1-6 (1997)

Yamamoto M.：“日本的除草剂和胆囊癌”毒理学评论。

KODAMA,KUNIKO et.al: "Geographic clustering patterns in mortality from biliary tract cancer in Japan" Jpn J Cancer Res. 89-1. 6-11 (1998)

KODAMA、KUNIKO 等人：“日本胆道癌死亡率的地理聚类模式”Jpn J Cancer Res。

Hori Y, Nakamura K, Yamamoto M, et al.: "Determination of free fatty acids in human bile by high-performance liquid chromatography" Annals of Clinical Biochemistry. 35(印刷中). (1998)

Hori Y、Nakamura K、Yamamoto M 等人：“通过高效液相色谱测定人胆汁中的游离脂肪酸”，《临床生物化学年鉴》35（出版中）。

Hori Y,Nakamura K,Yamamoto M, et al.: "Determination of free fatty acids in human bile by highperformance liquid chromatography" Annals of Clinical Biochemistry. 35・1(印刷中). (1997)

Hori Y、Nakamura K、Yamamoto M 等人：“通过高效液相色谱法测定人胆汁中的游离脂肪酸”，《临床生物化学年鉴》35·1（出版中）。