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International and Epidemiological Studies on the Causes of Gallbladder Cancer in Hungary

关于匈牙利胆囊癌病因的国际和流行病学研究

基本信息

批准号：
15406025
负责人：
YAMAMOTO Masaharu
金额：
$ 8万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

We have conducted epidemiological studies on gallbladder cancer (GBC) in Japan and Chile, where the ranks of mortality rate for GBC were the 5^<th> and the 1^<st>, respectively, in the world. As a next step, we studied the characteristics of GBC in Hungary since its rank was reported as the 2^<nd> in the world.(1)Case-Control Study of BBC : We collected 42 cases of GBC with GS from hospitals in Budapest, Hungary and 30 controls among outpatients of the hospital without GBC, but with GS. When a conditional logistic analysis was applied, the consumption of chili pepper and the age were found to be significantly related to the occurrence of GBC.(2)Genetic Polymorphisms of Cytochrome P450 1A1 : In order to clarify the individual differences in susceptibility to GBC, we investigated the frequency of the Msp1 and Ile-Val polymorphisms of the CYP1A1 gene, in 42 cases of GBC and 100 controls. There were no statistical differences in the frequencies of the mutant genotypes of Msp1 and Ile-Val polymorphism or their alleles between them.(3)Mutations of p53, K-ras codon 12 and Microsatellite Instability (MSI) : We collected 20 GBC in Hungary and 22 cases in Japan. Mutations of p53 were found in 6 out of 18 cases (33.3%) in Hungary and in 11 out of 22 cases (50.0%) in Japan, but no difference was found. Base-change spectra of mutation in Hungary were characterized as transition type, while in Japan they were all transversion type. There was no difference in K-ras mutations between two countries. The frequencies of MSI-H of GBC were 1/15 (6.7%) in Hungary and 8/19 (42.1%) in Japan. There was a significant difference between them.In conclusion, the consumption of chili pepper was also disclosed as a risk factor, as shown in Chile. In addition, the mutation spectra of p53 in Hungary were similar to those in Chile, but different from those in Japan.

我们在日本和智利进行了胆囊癌（GBC）的流行病学研究，这两个国家的GBC死亡率在世界上分别排名第5<th>和第1<st>。作为下一步，我们研究了匈牙利GBC的特点，因为它的排名被报道为世界第2^<nd>。（1）BBC病例对照研究：收集了42例来自匈牙利布达佩斯的GBC伴GS病例和30例来自该医院门诊的非GBC但伴GS的对照。经条件Logistic回归分析发现，辣椒的食用量和年龄与GBC的发生有显著相关。（2）细胞色素P450 1A 1基因多态性：为了探讨GBC易感性的个体差异，我们检测了42例GBC患者和100名对照者的CYP 1A 1基因Msp 1和Ile-Val多态性的频率。Msp 1和Ile-Val多态性的突变基因型及其等位基因频率在两组间差异无统计学意义。（3）p53、K-ras第12密码子突变和微卫星不稳定性（MSI）：收集了20例匈牙利GBC和22例日本GBC。匈牙利18例中有6例（33.3%）p53基因突变，日本22例中有11例（50.0%）p53基因突变，匈牙利p53基因突变为转换型，日本p53基因突变均为颠换型。两国间K-ras基因突变无差异。GBC的MSI-H频率在匈牙利为1/15（6.7%），在日本为8/19（42.1%）。总之，辣椒的消费也被认为是一个风险因素，如智利所示。此外，匈牙利的p53突变谱与智利相似，但与日本不同。