Histo-pathological Studies on the Effects of the Natural Coral Powdered as Pulp Capping Materials after Vital Dental Pulp Amputation

由于天然粉末珊瑚似乎是有效的牙髓盖材料，它们用X射线检测能量色散分析（Kevex 7000 Q）和X射线衍射（实验1）进行分析。在处理后的不同时间间隔，从组织病理学和超微结构上阐明用作盖髓材料的天然珊瑚粉对植入的周围组织（实验2）和对切断的狗牙髓（实验3）的影响。实验2将预先保存在3.0 × 7.0mm聚乙烯管中的两种天然珊瑚粉，即白色多孔珊瑚粉A和红色致密珊瑚粉B，经700 ℃加热30分钟，皮下植入36只大鼠背部组织中2 - 6个月。对于实验3，将加热的天然粉末珊瑚A和A放置在64颗狗牙齿的牙髓上，所述狗牙齿的牙髓的冠髓预先用圆棒（#5）切除，并且摘除器被消毒，并且进行处理 关于我们 常规治疗1 ~ 7周。作为对照，将氢氧化钙放置在12颗狗牙齿的牙髓上，而不是天然珊瑚粉相同的时间间隔。两种实验处理后不同时间，小心地取出周围组织，固定，石蜡或Epon 812包埋，常规切片，进行光镜、扫描或透射电镜观察，四种珊瑚皮下植入后周围结缔组织的炎症反应程度略有不同。特别是，在处理后的所有时间间隔，天然珊瑚A附近的组织比其他珊瑚附近的组织更牢固地被致密的纤维组织包裹。然而，在邻近所有类型的珊瑚植入凹面的组织中，炎症反应在所有时间间隔都保持不变。天然珊瑚A和热珊瑚A处理后1 ~ 3周，离体牙髓上均无明显牙本质桥形成，而5 ~ 7周时，50%的离体实验牙有骨样牙本质桥形成，而热珊瑚A处理后无骨样牙本质桥形成。在加热的珊瑚A和B以及天然珊瑚A和B中，观察到Ca、P、Mg、Fe和Al离子，但在A和B型珊瑚中Mg和Al的比例不同。少

As natural powdered corals appear to be effective as dental pulp capping materials, they were analyzed with x-ray detection energy dispersive analysis (Kevex 7000 Q) and with x-ray diffraction (experiment 1). The effects of the natural powdered coral for use as dental pulp capping material, on the surrounding tissues implanted (experiment 2) and on the dog dental pulp amputated (experiment 3) were elucidated histo-pathologically and ultrastructurally at various intervals after treatment. For experiment 2, two types of natural corals powdered namely, white and porous, A, and red and dense, B, and the two heated at 700ﾟC for 30 min, which were previously kept in the polyethylene tubes (3.0X7.0mm), were implanted subcutaneously into the dorsal tissues of 36 rats for 2 to 6 months. For experiment 3, natural powdered coral A and A heated were placed on the dog dental pulp of 64 teeth, coronal pulp of which was previously amputated with round bars(#5) and exscavators sterilized, and treated … More routinely for 1 to 7 weeks after treatment. As a control, calcium hydroxide was placed on the dental pulp of 12 dog teeth instead of the natural powdered coral for the same intervals. At various intervals after treatment for both experiments, the surrounding tissue was carefully removed, fixed, embedded in paraffin or Epon 812 and sectioned routinely for light- and scanning or transmission electron-microscopy.The degree of the inflammatory reaction in the surrounding connective tissues to 4 types of corals implanted subcutaneously was slightly different each other. In particular, the tissues adjacent to natural coral A were more strongly encapsulated with the dense fibrous tissues than those adjacent to the other coral at all intervals after treatment. However, in tissues adjacent to the concaved surfaces of all types of coral implanted, the inflammatory response remained at all intervals. The dent in bridge was not positivelyformed over the dental pulp amputated at 1 to 3 weeks after natural or heated coral A treatment, whereas at 5 to 7 weeks the bonelike dentin bridge was observed on 50% of theexperimental teeth treated with natural coral A, but not with heated coral A.The necro-sis of the dental pulp adjacent to natural and heated coral A were not observed over theexperimental periods. In the heated coral A and B, and natural coral A and B, Ca, P, Mg, Fe and Al ions were observed but the ratio of Mg and Al was different in the A and B types of corals.Natural coral A showed a characteristic peak as observed with calcite. Less